TA319226

antibody from OriGene

Targeting: HAUS8 HICE1, MGC20533, NY-SAR-48

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

TA319226

Provider

OriGene

Product name

Rabbit polyclonal anti-HICE1 antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal anti-HICE1 antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

HAUS8

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µg

Concentration

0.45 mg/mL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

WB of Rabbit Anti-Hice1 antibody. Lane 1: HeLa cell extracts of untransfected cells (-). Lane 2: transfected HeLa cell extracts with Flag X3-Hice1 WT (WT). Lane 3: transfected HeLa cell extracts with Flag X3-Hice1 S70A mutant (70A). Load: 35 ug per lane. Primary antibody: Hice1 antibody at 0.5 ug/mL for overnight at 4°C. Secondary antibody: IRDye800„? Conjugated Goat Anti-Rabbit IgG secondary antibody at 1:10,000 for 45 min at RT.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot of Rabbit anti-HICE1 antibody. Lane 1: 293T Null. Lane 2: 293T WT Hice1. Lane 3: 293T S70A Hice1. Load: 14 ?l per lane. Primary antibody: HICE1 antibody at 1.24 ug/mL overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 48 kDa, 48 kDa for HICE1. Other band(s): HICE1 splice variants and isoforms.

Validation comment

WB