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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-21331 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HICE1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: K562, HeLa. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.17 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Length-dependent poleward flux of sister kinetochore fibers promotes chromosome alignment.
Risteski P, Božan D, Jagrić M, Bosilj A, Pavin N, Tolić IM
Cell reports 2022 Aug 2;40(5):111169
Cell reports 2022 Aug 2;40(5):111169
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot to detect cellular Hice1 and Hice1-GFP expressed in human osteosarcoma U2OS cells (left image), and Hice1 upon siRNA treatment (right image), using HICE1 Polyclonal Antibody (Product # PA5-21331) at 1:1000 dilution. Nuclear matrix protein p84 is a loading control, blotted with a p84 antibody at a 1:5000 dilution. The HRP-conjµgated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HICE1 Polyclonal Antibody (Product # PA5-21331). Sample. A: His-Hice1 (2x). B: His-Hice1 (1x). 7.5% SDS PAGE. HICE1 Polyclonal Antibody (Product # PA5-21331) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of human osteosarcoma cell line U2OS, using HICE1 (Product # PA5-21331) antibody at 1:50-1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Human lymph tissue, using HICE1 (Product # PA5-21331) antibody at 1:50 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Human lymph tissue, using HICE1 (Product # PA5-21331) antibody at 1:50 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
