PA1-767A

antibody from Invitrogen Antibodies

Targeting: VAMP3 CEB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-767A

Provider

Invitrogen Antibodies

Product name

VAMP3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-767A detects vesicle-associated membrane protein 3 (VAMP-3)/cellubrevin in human, rat and mouse samples. PA1-767A has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~13 kDa protein representing VAMP-3 in HeLa cell lysates. PA1-767A immunogen is a sythetic peptide corresponding to residues M(1) S T G V P S G S S A A T G S N R R(18) C of mouse VAMP-3. This immunizing peptide (Cat. # PEP-145) is available for use in neutralization and control experiments.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Single bioengineered ncRNA molecule for dual-targeting toward the control of non-small cell lung cancer patient-derived xenograft tumor growth.

Petrek H, Yan Ho P, Batra N, Tu MJ, Zhang Q, Qiu JX, Yu AM
Biochemical pharmacology 2021 Jul;189:114392

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Recycling endosomes attach to the trans-side of Golgi stacks in Drosophila and mammalian cells.

Fujii S, Kurokawa K, Inaba R, Hiramatsu N, Tago T, Nakamura Y, Nakano A, Satoh T, Satoh AK
Journal of cell science 2020 Feb 26;133(4)

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SNAP23, Syntaxin4, and vesicle-associated membrane protein 7 (VAMP7) mediate trafficking of membrane type 1-matrix metalloproteinase (MT1-MMP) during invadopodium formation and tumor cell invasion.

Williams KC, McNeilly RE, Coppolino MG
Molecular biology of the cell 2014 Jul 1;25(13):2061-70

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Characterization of VAMP-2 in the lung: implication in lung surfactant secretion.

Wang P, Howard MD, Zhang H, Chintagari NR, Bell A, Jin N, Mishra A, Liu L
Cell biology international 2012 Sep;36(9):785-91

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of VAMP3 was performed by loading 25 µg of mouse brain (lane 1), rat brain (lane 2) and human brain (lane 3) lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a VAMP3 polyclonal antibody (Product # PA1-767A) at a dilution of 1:2000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~13 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Liver (Lane 3) and Rat Liver (Lane 4). The blot was probed with Anti-VAMP3 Polyclonal Antibody (Product # PA1-767A, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 15 kDa band corresponding to VAMP3 was observed across the tissue extracts tested except Mouse Liver and Rat Liver tested which is reported to be negative.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of VAMP3 (green) showing staining in the cytoplasm of C6 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a VAMP3 polyclonal antibody (Product # PA1-767A) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of VAMP3 (green) showing staining in the cytoplasm of Neuro-2a cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a VAMP3 polyclonal antibody (Product # PA1-767A) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of VAMP3 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a VAMP3 polyclonal antibody (Product # PA1-767A) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.