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Learn16081-1-AP
antibody from Invitrogen Antibodies
Targeting: WARS1
IFI53, IFP53, WARS
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 16081-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TrpRS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MPNSEPASL LELFNSIATQ GELVRSLKAG NASKDEIDSA VKMLVSLKMS YKAAAGEDYK ADCPPGNPAP TSNHGPDATE AEEDFVDPWT VQTSSAKGID YDKLIVRFGS SKIDKELINR IERATGQRPH HFLRRGIFFS HRDMNQVLDA YENKKPFYLY TGRGPSSEAM HVGHLIPFIF TKWLQDVFNV PLVIQMTDDE KYLWKDLTLD QAYSYAVENA KDIIACGFDI NKTFIFSDLD YMGMSSGFYK NVVKIQKHVT FNQVKGIFGF TDSDCIGKIS FPAIQAAPSF SNSFPQIFRD RTDIQCLIPC AIDQDPYFRM TRDVAPRIGY PKPALLHSTF FPALQGAQTK MSASDPNSSI FLTDTAKQI (1-368 aa encoded by BC017489 )
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 16081-1-AP (TrpRS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 16081-1-AP (TrpRS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 16081-1-AP (TrpRS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 16081-1-AP (TrpRS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using WARS antibody 16081-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 16081-1-AP (TrpRS antibody at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 16081-1-AP (TrpRS antibody at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary tumor using 16081-1-AP (TrpRS antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary tumor using 16081-1-AP (TrpRS antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HepG2 cells were stained with 0.2ug TrpRS antibody (16081-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-TrpRS (IP:16081-1-AP, 4ug; Detection:16081-1-AP 1:500) with HepG2 cells lysate 3000ug.
