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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Other assay [1]
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- Product number
- PA5-88785 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEMG2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: HGKSQNQVRI PSQAQEYGHK ENKISYQSSS TEERRLNSGE KDVQKGVSKG SISIQTEEKI HGKSQNQVTI PSQDQEHGHK ENKMSYQSSS TEERRLNYGG KSTQKDVSQS SISFQIEKLV EGKSQIQTPN PNQDQWSGQN AKGKSGQSAD SKQDLLSHEQ KGRYKQESSE SHNIVITEHE VAQDDHLTQQ YNEDRNPIST; Positive Samples: 293T, HeLa, Mouse testis
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.34 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Baicalin Attenuates Continuous Activation of β-Catenin Induced by Lipopolysaccharide (LPS) and Depression Complicated by Infertility in Male Rats.
Fan R, Dai F, Zhang C, Zhou L, Qiu X, Li Y
Evidence-based complementary and alternative medicine : eCAM 2022;2022:2112359
Evidence-based complementary and alternative medicine : eCAM 2022;2022:2112359
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using SEMG2 Polyclonal antibody (Product # PA5-88785) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SEMG2 in extracts of various cell lines using SEMG2 Polyclonal Antibody (Product # PA5-88785) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SEMG2 in extracts of HeLa (25 µg per lane). Samples were incubated in SEMG2 polyclonal antibody (Product # PA5-88785) using a dilution of 1:3,000 followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 BA alleviated sterility by regulating beta -catenin. (a) WB was used to measure the levels of MIS in rat testicular tissue. (b) WB was performed to detect the expression of ANXA2, APP, SEMG1, and SEMG2 in rat seminal plasma proteins. (c) TUNEL was applied to measure the apoptosis rate of germ cells. Scale bar = 25 mu m. (d) The number of mating plugs in each group. (e) The pregnancy rate of female rats mated with rats in each group. (f) Pregnancy time of rats in each group. (g) Sperm motility of rats in each group. * P < 0.05 vs control group; # P < 0.05 vs. CUMS + LPS group.
