15168-1-AP

antibody from Invitrogen Antibodies

Targeting: SIGMAR1 OPRS1, SR-BP1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

15168-1-AP

Provider

Invitrogen Antibodies

Product name

SIGMAR1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: SEYVLLFGT ALGSRGHSGR YWAEISDTII SGTFHQWREG TTKSEVFYPG ETVVHGPGEA TAVEWGPNTW MVEYGRGVIP STLAFALADT VFSTQDFLTL FYTLRSYARG LRLELTTYLF GQDP (101-223 aa encoded by B C004899)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.3 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15168-1-AP (SIGMAR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse colon tissue were subjected to SDS PAGE followed by western blot with 15168-1-AP (SIGMAR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 15168-1-AP (SIGMAR1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

COLO 320 cells were subjected to SDS PAGE followed by western blot with 15168-1-AP (SIGMAR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 15168-1-AP ( SIGMAR1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 15168-1-AP ( SIGMAR1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using (Product # 15168-1-AP) (SIGMAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat brain tissue slide using 15168-1-AP (SIGMAR1 antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat brain tissue slide using 15168-1-AP (SIGMAR1 antibody) at dilution of 1:200 (under 40x lens).