Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-60426 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-60426, RRID:AB_2269264
- Product name
- Rabbit Polyclonal RBM27 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RBM27 Antibody [NB100-60426] - Detection of Human RBM27 on HeLa whole cell lysate using NB100-60426. RBM27 was also immunoprecipitated by rabbit anti-RBM27 antibody NB100-60427.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RBM27 Antibody [NB100-60426] - Detection of Human and Mouse RBM27 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-RBM27 antibody NB100-60426 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RBM27 Antibody [NB100-60426] - Detection of human RBM27 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RBM27 antibody NB100-60426 used for IP at 6 ug per reaction. RBM27 was also immunoprecipitated by another rabbit anti-RBM27 antibody. For blotting immunoprecipitated RBM27, NB100-60426 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: RBM27 Antibody [NB100-60426] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- RBM27 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB