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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- GTX80966 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX80966, RRID:AB_11174765
- Product name
- ALOX12 antibody, C-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of ALOX12 Antibody (C-term) (GTX80966) in K562 cell line lysates (35μg/lane). ALOX12 (arrow) was detected using the purified Pab.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of ALOX12 Antibody (C-term) (GTX80966) with 293 cell followed by Alexa FluorºS?488-conjμgated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human skin tissue reacted with ALOX12 Antibody (C-term) (GTX80966), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ALOX12 Antibody (C-term) (GTX80966) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.
