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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [7]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- GTX113164 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113164, RRID:AB_1949973
- Product name
- Choline Acetyltransferase antibody [N1N3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: H1299 7.5% SDS PAGE GTX113164 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- Choline Acetyltransferase antibody [N1N3] detects Choline Acetyltransferase protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Choline Acetyltransferase antibody [N1N3] detects Choline Acetyltransferase protein by western blot analysis. Rat tissue extracts (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CHAT(GTX113164) antibody at 1:200 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Choline Acetyltransferase antibody [N1N3] detects Choline Acetyltransferase protein by immunofluorescent analysis.Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: Choline Acetyltransferase protein stained by Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Choline Acetyltransferase antibody [N1N3] detects Choline Acetyltransferase protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:400.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Choline Acetyltransferase antibody [N1N3] detects Choline Acetyltransferase protein in the amacrine cells by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: Choline Acetyltransferase protein stained by Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:250.Red: Protein kinase C alpha staining.Blue: Fluoroshield with DAPI (GTX30920).
