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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 100-401-D16 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#100-401-D16, RRID:AB_11183445
- Product name
- Anti-ChAT (Choline Acetyltransferase) (Rabbit) Antibody - 100-401-D16
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of Rabbit Anti-ChAT antibody. Lane 1: rat brain. Lane 2: none. Load: 10 µg per lane. Primary antibody: Choline Acetyltransferase antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~68/70k,~68/70k for choline acetyltransferase protein. Other band(s): ChAT splice variants and isoforms.
- Validation comment
- Western Blot
