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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- 600-101-D16 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-101-D16, RRID:AB_11183124
- Product name
- Anti-ChAT (Choline Acetyltransferase) (Goat) Antibody - 600-101-D16
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references Lhx6-positive GABA-releasing neurons of the zona incerta promote sleep.
Liu K, Kim J, Kim DW, Zhang YS, Bao H, Denaxa M, Lim SA, Kim E, Liu C, Wickersham IR, Pachnis V, Hattar S, Song J, Brown SP, Blackshaw S
Nature 2017 Aug 31;548(7669):582-587
Nature 2017 Aug 31;548(7669):582-587
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of Goat Anti-Choline Acetyltransferase Antibody. Lane 1: rat brain lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: ChAT antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800 goat secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~70kDa/~70kDa for ChAT. Other band(s): none.
- Validation comment
- Western Blot
