Antibody data
- Antibody Data
- Antigen structure
- References [23]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
Reference
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- Product number
- 20747-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#20747-1-AP, RRID:AB_10898169
- Product name
- CHAT antibody
- Antibody type
- Polyclonal
- Description
- CHAT antibody (Cat. #20747-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Dopamine receptor 2 downregulation and brain-derived neurotrophic factor upregulation in the paraventricular nucleus are correlated with brown adipose tissue thermogenesis in rats with bilateral substantia nigra lesions.
BDNF-overexpressing human umbilical cord mesenchymal stem cell-derived motor neurons improve motor function and prolong survival in amyotrophic lateral sclerosis mice.
A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.
Premigratory neural crest stem cells generate enteric neurons populating the mouse colon and regulating peristalsis in tissue-engineered intestine.
Synergistic Effect of the Long-Term Overexpression of Bcl-2 and BDNF Lentiviral in Cell Protecting against Death and Generating TH Positive and CHAT Positive Cells from MSC.
Mixture of Phlorotannin and Fucoidan from Ecklonia cava Prevents the Aβ-Induced Cognitive Decline with Mitochondrial and Cholinergic Activation.
Ecklonia cava Attenuates PM2.5-Induced Cognitive Decline through Mitochondrial Activation and Anti-Inflammatory Effect.
Electroacupuncture improves cardiac function and reduces infarct size by modulating cardiac autonomic remodeling in a mouse model of myocardial ischemia.
Intravenously Administered, Retinoid Activating Nanoparticles Increase Lifespan and Reduce Neurodegeneration in the SOD1G93A Mouse Model of ALS.
HJ22, a Novel derivative of piperine, Attenuates ibotenic acid-induced cognitive impairment, oxidativestress, apoptosis and inflammation via inhibiting the protein-protein interaction of Keap1-Nrf2.
LSM12-EPAC1 defines a neuroprotective pathway that sustains the nucleocytoplasmic RAN gradient.
Expression of Dopamine Receptors in the Lateral Hypothalamic Nucleus and Their Potential Regulation of Gastric Motility in Rats With Lesions of Bilateral Substantia Nigra.
Melatonin alleviates cognition impairment by antagonizing brain insulin resistance in aged rats fed a high-fat diet.
Partially Hydrolyzed Guar Gum Attenuates d-Galactose-Induced Oxidative Stress and Restores Gut Microbiota in Rats.
Young Blood Rescues the Cognition of Alzheimer's Model Mice by Restoring the Hippocampal Cholinergic Circuit.
Brain-derived neurotrophic factor modified human umbilical cord mesenchymal stem cells-derived cholinergic-like neurons improve spatial learning and memory ability in Alzheimer's disease rats.
Enteric glial cell activation protects enteric neurons from damage due to diabetes in part via the promotion of neurotrophic factor release.
Neuron-Specific HuR-Deficient Mice Spontaneously Develop Motor Neuron Disease.
Tail Nerve Electrical Stimulation and Electro-Acupuncture Can Protect Spinal Motor Neurons and Alleviate Muscle Atrophy after Spinal Cord Transection in Rats.
Modeling the phenotype of spinal muscular atrophy by the direct conversion of human fibroblasts to motor neurons.
Intestinal injury in the rat model of 17α-ethynylestradiol-induced intrahepatic cholestasis.
Myocardial Infarction Causes Transient Cholinergic Transdifferentiation of Cardiac Sympathetic Nerves via gp130.
Alleviating effects of Bushen-Yizhi formula on ibotenic acid-induced cholinergic impairments in rat.
Zhang Y, Zhou L, Lian H, Zhang Y, Tong S, Wang Z
Journal of chemical neuroanatomy 2021 Nov;117:102016
Journal of chemical neuroanatomy 2021 Nov;117:102016
BDNF-overexpressing human umbilical cord mesenchymal stem cell-derived motor neurons improve motor function and prolong survival in amyotrophic lateral sclerosis mice.
Wang J, Hu W, Feng Z, Feng M
Neurological research 2021 Mar;43(3):199-209
Neurological research 2021 Mar;43(3):199-209
A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.
Wu X, Sheng H, Wang L, Xia P, Wang Y, Yu L, Lv W, Hu J
Aging 2021 Mar 26;13(7):10034-10057
Aging 2021 Mar 26;13(7):10034-10057
Premigratory neural crest stem cells generate enteric neurons populating the mouse colon and regulating peristalsis in tissue-engineered intestine.
Yuan H, Hu H, Chen R, Mu W, Wang L, Li Y, Chen Y, Ding X, Xi Y, Mao S, Jiang M, Chen J, He Y, Wang L, Dong Y, Tou J, Chen W
Stem cells translational medicine 2021 Jun;10(6):922-938
Stem cells translational medicine 2021 Jun;10(6):922-938
Synergistic Effect of the Long-Term Overexpression of Bcl-2 and BDNF Lentiviral in Cell Protecting against Death and Generating TH Positive and CHAT Positive Cells from MSC.
Borkowska P, Zielinska A, Paul-Samojedny M, Stojko R, Kowalski J
International journal of molecular sciences 2021 Jun 30;22(13)
International journal of molecular sciences 2021 Jun 30;22(13)
Mixture of Phlorotannin and Fucoidan from Ecklonia cava Prevents the Aβ-Induced Cognitive Decline with Mitochondrial and Cholinergic Activation.
Han HJ, Park SK, Kang JY, Kim JM, Yoo SK, Kim DO, Kim GH, Heo HJ
Marine drugs 2021 Jul 29;19(8)
Marine drugs 2021 Jul 29;19(8)
Ecklonia cava Attenuates PM2.5-Induced Cognitive Decline through Mitochondrial Activation and Anti-Inflammatory Effect.
Park SK, Kang JY, Kim JM, Kim HJ, Heo HJ
Marine drugs 2021 Feb 27;19(3)
Marine drugs 2021 Feb 27;19(3)
Electroacupuncture improves cardiac function and reduces infarct size by modulating cardiac autonomic remodeling in a mouse model of myocardial ischemia.
Lu SF, Wang JM, Yuan J, Yang WX, Chen LY, Zhang T, Jing XY, Zhuang Y, Zhang CS, Fu SP, Yu ML
Acupuncture in medicine : journal of the British Medical Acupuncture Society 2021 Dec;39(6):681-690
Acupuncture in medicine : journal of the British Medical Acupuncture Society 2021 Dec;39(6):681-690
Intravenously Administered, Retinoid Activating Nanoparticles Increase Lifespan and Reduce Neurodegeneration in the SOD1G93A Mouse Model of ALS.
Medina DX, Chung EP, Teague CD, Bowser R, Sirianni RW
Frontiers in bioengineering and biotechnology 2020;8:224
Frontiers in bioengineering and biotechnology 2020;8:224
HJ22, a Novel derivative of piperine, Attenuates ibotenic acid-induced cognitive impairment, oxidativestress, apoptosis and inflammation via inhibiting the protein-protein interaction of Keap1-Nrf2.
Yang X, Ji J, Liu C, Zhou M, Li H, Ye S, Hu Q
International immunopharmacology 2020 Jun;83:106383
International immunopharmacology 2020 Jun;83:106383
LSM12-EPAC1 defines a neuroprotective pathway that sustains the nucleocytoplasmic RAN gradient.
Lee J, Park J, Kim JH, Lee G, Park TE, Yoon KJ, Kim YK, Lim C
PLoS biology 2020 Dec;18(12):e3001002
PLoS biology 2020 Dec;18(12):e3001002
Expression of Dopamine Receptors in the Lateral Hypothalamic Nucleus and Their Potential Regulation of Gastric Motility in Rats With Lesions of Bilateral Substantia Nigra.
Yang YL, Ran XR, Li Y, Zhou L, Zheng LF, Han Y, Cai QQ, Wang ZY, Zhu JX
Frontiers in neuroscience 2019;13:195
Frontiers in neuroscience 2019;13:195
Melatonin alleviates cognition impairment by antagonizing brain insulin resistance in aged rats fed a high-fat diet.
Xu J, Gao H, Zhang L, Rong S, Yang W, Ma C, Chen M, Huang Q, Deng Q, Huang F
Journal of pineal research 2019 Sep;67(2):e12584
Journal of pineal research 2019 Sep;67(2):e12584
Partially Hydrolyzed Guar Gum Attenuates d-Galactose-Induced Oxidative Stress and Restores Gut Microbiota in Rats.
Liu X, Wu C, Han D, Liu J, Liu H, Jiang Z
International journal of molecular sciences 2019 Sep 30;20(19)
International journal of molecular sciences 2019 Sep 30;20(19)
Young Blood Rescues the Cognition of Alzheimer's Model Mice by Restoring the Hippocampal Cholinergic Circuit.
Xia E, Xu F, Hu C, Kumal JPP, Tang X, Mao D, Li Y, Wu D, Zhang R, Wu S, Sun L
Neuroscience 2019 Oct 1;417:57-69
Neuroscience 2019 Oct 1;417:57-69
Brain-derived neurotrophic factor modified human umbilical cord mesenchymal stem cells-derived cholinergic-like neurons improve spatial learning and memory ability in Alzheimer's disease rats.
Hu W, Feng Z, Xu J, Jiang Z, Feng M
Brain research 2019 May 1;1710:61-73
Brain research 2019 May 1;1710:61-73
Enteric glial cell activation protects enteric neurons from damage due to diabetes in part via the promotion of neurotrophic factor release.
Luo P, Liu D, Li C, He WX, Zhang CL, Chang MJ
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society 2018 Oct;30(10):e13368
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society 2018 Oct;30(10):e13368
Neuron-Specific HuR-Deficient Mice Spontaneously Develop Motor Neuron Disease.
Sun K, Li X, Chen X, Bai Y, Zhou G, Kokiko-Cochran ON, Lamb B, Hamilton TA, Lin CY, Lee YS, Herjan T
Journal of immunology (Baltimore, Md. : 1950) 2018 Jul 1;201(1):157-166
Journal of immunology (Baltimore, Md. : 1950) 2018 Jul 1;201(1):157-166
Tail Nerve Electrical Stimulation and Electro-Acupuncture Can Protect Spinal Motor Neurons and Alleviate Muscle Atrophy after Spinal Cord Transection in Rats.
Zhang YT, Jin H, Wang JH, Wen LY, Yang Y, Ruan JW, Zhang SX, Ling EA, Ding Y, Zeng YS
Neural plasticity 2017;2017:7351238
Neural plasticity 2017;2017:7351238
Modeling the phenotype of spinal muscular atrophy by the direct conversion of human fibroblasts to motor neurons.
Zhang QJ, Li JJ, Lin X, Lu YQ, Guo XX, Dong EL, Zhao M, He J, Wang N, Chen WJ
Oncotarget 2017 Feb 14;8(7):10945-10953
Oncotarget 2017 Feb 14;8(7):10945-10953
Intestinal injury in the rat model of 17α-ethynylestradiol-induced intrahepatic cholestasis.
Chang MJ, Xu YJ, He WX, Zhang CL, Li XP, Liu D
Journal of digestive diseases 2016 Nov;17(11):756-763
Journal of digestive diseases 2016 Nov;17(11):756-763
Myocardial Infarction Causes Transient Cholinergic Transdifferentiation of Cardiac Sympathetic Nerves via gp130.
Olivas A, Gardner RT, Wang L, Ripplinger CM, Woodward WR, Habecker BA
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Jan 13;36(2):479-88
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Jan 13;36(2):479-88
Alleviating effects of Bushen-Yizhi formula on ibotenic acid-induced cholinergic impairments in rat.
Hou XQ, Zhang L, Yang C, Rong CP, He WQ, Zhang CX, Li S, Su RY, Chang X, Qin JH, Chen YB, Xian SX, Wang Q
Rejuvenation research 2015 Apr;18(2):111-27
Rejuvenation research 2015 Apr;18(2):111-27
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 20747-1-AP(CHAT antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using CHAT antibody 20747-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human stomach using 20747-1-AP(CHAT antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human stomach using 20747-1-AP(CHAT antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue