Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [7]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
- Other assay [1]
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- Product number
- PA5-29653 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ChAT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: IMR32, mouse brain, rat brain, DDDDK-tagged CHAT-transfected 293T.
- Concentration
- 1.23 mg/mL
Submitted references Focal impaired awareness seizures in a rodent model: A functional anatomy.
Targeting the tamoxifen receptor within sodium channels to block osteoarthritic pain.
Angiopoietin-1 Knockout Mice as a Genetic Model of Open-Angle Glaucoma.
Adotevi N, Kapur J
Epilepsia open 2022 Mar;7(1):110-123
Epilepsia open 2022 Mar;7(1):110-123
Targeting the tamoxifen receptor within sodium channels to block osteoarthritic pain.
McCollum MM, Larmore M, Ishihara S, Ng LCT, Kimura LF, Guadarrama E, Ta MC, Vien TN, Frost GB, Scheidt KA, Miller RE, DeCaen PG
Cell reports 2022 Aug 23;40(8):111248
Cell reports 2022 Aug 23;40(8):111248
Angiopoietin-1 Knockout Mice as a Genetic Model of Open-Angle Glaucoma.
Thomson BR, Grannonico M, Liu F, Liu M, Mendapara P, Xu Y, Liu X, Quaggin SE
Translational vision science & technology 2020 Mar;9(4):16
Translational vision science & technology 2020 Mar;9(4):16
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CHAT using 30 µg of H1299 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a CHAT polyclonal antibody (Product # PA5-29653) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Choline Acetyltransferase in Rat tissue extracts (50 µg). Samples was separated by 7.5% SDS-PAGE and the membrane was probed with Choline Acetyltransferase Polyclonal antibody (Product # PA5-29653) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Choline Acetyltransferase in Various whole cell extracts (30 µg). Samples were separated by 7.5% SDS-PAGE and the membrane was probed with Choline Acetyltransferase Polyclonal antibody (Product # PA5-29653) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ChAT Polyclonal Antibody (Product # PA5-29653). Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ChAT Polyclonal Antibody (Product # PA5-29653) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-ChAT Polyclonal Antibody (Product # PA5-29653) and a 70 kDa band corresponding to acetyl CoA; choline O-acetyltransferase was observed in relevant tissue models tested. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Kidney (Lane 3) and Rat Kidney (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Uncharacterized bands (*) were observed at ~35 kDa and ~28 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ChAT was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ChAT Polyclonal Antibody (Product # PA5-29653) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ChAT was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ChAT Polyclonal Antibody (Product # PA5-29653) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ChAT was performed in DIV14 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: ChAT Polyclonal Antibody (Product # PA5-29653) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of ChAT was performed in frozen sectioned adult mouse retina tissue using ChAT Polyclonal Antibody (Product # PA5-29653) at a dilution of 1:250 (Green). Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ChAT was performed in paraffin-embedded mouse colon tissue using ChAT Polyclonal Antibody (Product # PA5-29653) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ChAT was performed in paraffin-embedded mouse intestine tissue using ChAT Polyclonal Antibody (Product # PA5-29653) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of ChAT was performed in frozen sectioned adult mouse retina tissue using ChAT Polyclonal Antibody (Product # PA5-29653) at a dilution of 1:250 (Green). Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChAT Polyclonal Antibody detects Choline Acetyltransferase protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse spinal cord. Red: Choline Acetyltransferase stained by ChAT Polyclonal Antibody (Product # PA5-29653) diluted at 1:300. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChAT Polyclonal Antibody detects Choline Acetyltransferase protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: Choline Acetyltransferase stained by ChAT Polyclonal Antibody (Product # PA5-29653) diluted at 1:1,000. Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 3 Multiple cortical structures are activated during a focal impaired awareness seizure. (A) Coronal sections at bregma 0.13 mm showing TRAPed neurons in the anterior cingulate cortex during a seizure (top panel) and in controls (bottom panel) in merged images showing NeuN immunolabels in green and TdT + cells in red. (B) Coronal sections at bregma -1.91 mm showing TRAPed neurons in the retrosplenial and parietal cortices during a seizure (top panel) and in controls (bottom panel). (C) Horizontal sections at bregma -1.68 mm showing TdT activation in the frontal, orbital, motor, somatosensory, and visual cortices with (left panel) or without (right panel) a seizure. Merged images show TdT + cells (red) and ChAT immunolabeling (green). (D) Coronal sections at bregma 1.09 mm showing TRAPed neurons in the piriform (layers 1, 2, and 3) and endopiriform cortex. (E) A greater number of TdT-labeled cells were present in the ACC during a behavioral grade 2 seizure compared with controls (control 166 +- 10, seizure 274 +- 26; n = 5 mice per group, t( 5.22) = 5.65, P = .002). (F) More piriform cortex cell body layer II neurons were TdT-labeled compared with controls (control 100 +- 15, seizure 208 +- 33; n = 5 mice per group, t (5.56) = 2.91, P = .028). (G) Coronal sections at bregma 2.09 mm showing TRAPed neurons in motor and insular cortices, and olfactory nucleus. Regions marked are ac, anterior commissure; ACC, anterior cingulate cortex; AI, agranular insular cortex; AO, anter