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- Antibody Data
- Antigen structure
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- Immunohistochemistry [2]
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- Product number
- PA5-58478 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- METTL7B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: RVLRPGGVLF FWEHVAEPYG SWAFMWQQVF EPTWKHIGDG CCLTRETWKD LENAQFSEIQ MERQPPPLKW LPVGPHIMGK AV
- Concentration
- 0.2 mg/mL
Submitted references Human METTL7B is an alkyl thiol methyltransferase that metabolizes hydrogen sulfide and captopril.
Maldonato BJ, Russell DA, Totah RA
Scientific reports 2021 Mar 1;11(1):4857
Scientific reports 2021 Mar 1;11(1):4857
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of METTL7B in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a METTL7B Polyclonal Antibody (Product # PA5-58478).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of METTL7B in human cell lines Caco-2 and A-431 using a METTL7B Polyclonal Antibody (Product # PA5-58478). Corresponding METTL7B RNA-seq data are presented for the same cell lines. Loading control: Anti-PARP1.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of METTL7B in human cell line U-251 MG shows positivity in vesicles & microtubules. Samples were probed using a METTL7B Polyclonal Antibody (Product # PA5-58478).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of METTL7B in human epididymis using METTL7B Polyclonal Antibody (Product # PA5-58478) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of METTL7B in human endometrium using METTL7B Polyclonal Antibody (Product # PA5-58478) shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Overexpression of the METTL7B gene in HeLa cells: ( A ) RT-PCR data measuring METTL7B gene expression in HeLa cells treated with a METTL7B overexpression plasmid compared to control cells transfected with an empty expression vector. ( B ) S -methyl captopril measured 24 h following the addition of captopril (500 uM) to HeLa cells treated with METTL7B overexpression plasmid compared to control cells. ( C ) FLAG-tagged METTL7B expression is only observed in HeLa cells treated with the METTL7B overexpression plasmid and not in controls. Western blot using an anti-FLAG antibody confirmed the expression of METTL7B in HeLa cells. Full gel images are available in Supplementary Fig. 1 . All data is presented as the mean +- s.d. Individual data points are plotted from two separate experiments. Significance was determined using unpaired two-tailed t test. **** P < 0.0001.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Purified His-GST-METTL7B: (Lane 1) SDS-PAGE silver stain of a representative gel showing purified His-GST-METTL7B (A). Gel lanes were loaded with a 1 µg total protein as determined by A 280. (Lane 2) Anti-GST antibody western blot of purified His-GST-METTL7B (1 µg total protein). (Lane 3) Western blot using anti-METTL7B antibody of purified His-GST-METTL7B (A) using 0.1 µg total protein. Molecular weight markers are shown to the left. The lower molecular bands, marked by letters B and C, are fusion protein fragments containing the dual His-GST affinity tag.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 SDS-PAGE and western blot analysis of purified His-GST-METTL7B-D98A: (Lane 1) SDS-PAGE silver stain of a representative gel showing purified His-GST-METTL7B-D98A (A) (4.2 µg total protein) (Lane 2) Anti-GST antibody western blot of purified His-GST-METTL7B-D98A (3 µg total protein). (Lane 3) Anti-METTL7B antibody western blot of purified His-GST-METTL7B-D98A (4.3 µg total protein). Molecular weight markers are shown to the left. Upper bands, above 55 kDa, are co-purifying chaperone proteins as determined by proteomic analysis (data not shown). Lower molecular bands, marked by letters B and C, are fusion protein fragments containing the dual His-GST affinity tag.
