Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA1-16544 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IREB2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody has 94% sequence homology with human, primate and rat.
- Concentration
- 1.15 mg/mL
Submitted references Manganese causes neurotoxic iron accumulation via translational repression of amyloid precursor protein and H-Ferritin.
Tumor-initiating cells of breast and prostate origin show alterations in the expression of genes related to iron metabolism.
Venkataramani V, Doeppner TR, Willkommen D, Cahill CM, Xin Y, Ye G, Liu Y, Southon A, Aron A, Au-Yeung HY, Huang X, Lahiri DK, Wang F, Bush AI, Wulf GG, Ströbel P, Michalke B, Rogers JT
Journal of neurochemistry 2018 Dec;147(6):831-848
Journal of neurochemistry 2018 Dec;147(6):831-848
Tumor-initiating cells of breast and prostate origin show alterations in the expression of genes related to iron metabolism.
Rychtarcikova Z, Lettlova S, Tomkova V, Korenkova V, Langerova L, Simonova E, Zjablovskaja P, Alberich-Jorda M, Neuzil J, Truksa J
Oncotarget 2017 Jan 24;8(4):6376-6398
Oncotarget 2017 Jan 24;8(4):6376-6398
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of IRP2 in mouse liver lysate using PA1-16544.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot detection of IRP2 in RAW 264.7 lysate using PA1-16544. Lane 1: Lot B Lane 2: Lot C
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IREB2 in Raw macrophages (mouse). Samples were incubated in IREB2 polyclonal antibody (Product # PA1-16544 using a dilution of 1:500. Lane 1: untreated; Lane 2: treated overnight with 100mM DFO; Lane 3: 100mM hemin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IREB2 in RAW 264.7 lysate. Samples in Lane 1: Lot B Lane 2: Lot C were incubated in IREB2 polyclonal antibody (Product # PA1-16544.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IREB2 in mouse liver lysate. Sample was incubated in IREB2 polyclonal antibody (Product # PA1-16544).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of IREB2 in formalin fixed paraffin-embedded (FFPE) human placenta. Samples were incubated in IREB2 polyclonal antibody (Product # PA1-16544) using a dilution of 1:300. Bond Rx autostainer (Leica Biosystems). The assay involved 30 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 9.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 15 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Cytoplasmic staining was observed in villi of endothelial cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
