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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- NB100-1798 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1798, RRID:AB_10000490
- Product name
- Rabbit Polyclonal IRP2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.15 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Iron accumulation in senescent cells is coupled with impaired ferritinophagy and inhibition of ferroptosis.
Mitochondrial iron chelation ameliorates cigarette smoke-induced bronchitis and emphysema in mice.
Regulation of iron metabolism in Hamp (-/-) mice in response to iron-deficient diet.
Alterations in renal iron metabolism caused by a copper/zinc-superoxide dismutase deficiency.
Identification of nonferritin mitochondrial iron deposits in a mouse model of Friedreich ataxia.
Neurons lacking iron regulatory protein-2 are highly resistant to the toxicity of hemoglobin.
Masaldan S, Clatworthy SAS, Gamell C, Meggyesy PM, Rigopoulos AT, Haupt S, Haupt Y, Denoyer D, Adlard PA, Bush AI, Cater MA
Redox biology 2018 Apr;14:100-115
Redox biology 2018 Apr;14:100-115
Mitochondrial iron chelation ameliorates cigarette smoke-induced bronchitis and emphysema in mice.
Cloonan SM, Glass K, Laucho-Contreras ME, Bhashyam AR, Cervo M, Pabón MA, Konrad C, Polverino F, Siempos II, Perez E, Mizumura K, Ghosh MC, Parameswaran H, Williams NC, Rooney KT, Chen ZH, Goldklang MP, Yuan GC, Moore SC, Demeo DL, Rouault TA, D'Armiento JM, Schon EA, Manfredi G, Quackenbush J, Mahmood A, Silverman EK, Owen CA, Choi AM
Nature medicine 2016 Feb;22(2):163-74
Nature medicine 2016 Feb;22(2):163-74
Regulation of iron metabolism in Hamp (-/-) mice in response to iron-deficient diet.
Masaratana P, Patel N, Latunde-Dada GO, Vaulont S, Simpson RJ, McKie AT
European journal of nutrition 2013 Feb;52(1):135-43
European journal of nutrition 2013 Feb;52(1):135-43
Alterations in renal iron metabolism caused by a copper/zinc-superoxide dismutase deficiency.
Yoshihara D, Fujiwara N, Kato S, Sakiyama H, Eguchi H, Suzuki K
Free radical research 2012 Jun;46(6):750-7
Free radical research 2012 Jun;46(6):750-7
Identification of nonferritin mitochondrial iron deposits in a mouse model of Friedreich ataxia.
Whitnall M, Suryo Rahmanto Y, Huang ML, Saletta F, Lok HC, Gutiérrez L, Lázaro FJ, Fleming AJ, St Pierre TG, Mikhael MR, Ponka P, Richardson DR
Proceedings of the National Academy of Sciences of the United States of America 2012 Dec 11;109(50):20590-5
Proceedings of the National Academy of Sciences of the United States of America 2012 Dec 11;109(50):20590-5
Neurons lacking iron regulatory protein-2 are highly resistant to the toxicity of hemoglobin.
Regan RF, Chen M, Li Z, Zhang X, Benvenisti-Zarom L, Chen-Roetling J
Neurobiology of disease 2008 Aug;31(2):242-9
Neurobiology of disease 2008 Aug;31(2):242-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IRP2 Antibody [NB100-1798] - Detection of IRP2 in RAW 264.7 lysate using NB100-1798. Lane 1: Lot B Lane 2: Lot C
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IRP2 Antibody [NB100-1798] - Detection of IRP2 in mouse liver lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IRP2 Antibody [NB100-1798] - Raw macrophages (mouse) were either left untreated (lane 1) or treated overnight with 100mM DFO (lane 2) or 100mM hemin (lane 3). Proteins extracts were subjected to western blotting using IRP2 or b-actin antibodies diluted 1:500 in TBS-Tween.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: IRP2 Antibody [NB100-1798] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human placenta using 1:300 conc. of IRP2 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 30 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 9.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 15 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Cytoplasmic staining was observed in villi of endothelial cells.
