600-401-MK7

antibody from Invitrogen Antibodies

Targeting: SLC18A3 VACHT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-MK7

Provider

Invitrogen Antibodies

Product name

VAChT Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Concentration

0.9 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot of Rabbit Anti-VAChT Antibody. Molecular Weight Marker: Opal Pre-stained (p/n MB-210-0500). Lane 1: Rat brain, post natal, Whole Cell Lysate (p/n W12-001-MQ6). Primary Antibody: Anti-VAChT Antibody at 1:1000 overnight at 4°C. Secondary Antibody: Donkey Anti-Rabbit IgG HRP (p/n 611-703-127) at 1:70,000 for 1hr at RT. Blocking: BlockOut Universal Buffer (p/n MB-073). Expected: ~57 kDa, protein glycosylation.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of Rabbit Anti-VAChT Antibody. Cell Line: Post-natal rat pup (PND1) heterogeneous brain cells. Primary Antibody: Rabbit Anti-VAChT Antibody at 15µg/mL overnight at 4°C. Counterstain Antibody: Donkey Anti-Rabbit IgG DyLight™ 488 Conjugated (p/n 611-741-127) at 5µg/mL for 1hr at RT. Fixative: 4% PFA. Permeabilization: 0.3% Triton X-100. Nuclear Counter Stain: DAPI. Image: A) DAPI (blue). B) Anti-VAChT (green) and Secondary. C) Merge of A+B images. D) Secondary only. Localization: predicted membrane, Golgi apparatus. Antibody detecting dendrites and axons and specific organelles in the nucleus (nucleolus). Staining correspondent to confirmed Slc18a3 staining in mouse brain P7 Gensat project: http://www.gensat.org/imagenavigator.jsp?imageID=66611

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of Rabbit Anti-VAChT Antibody. Tissue: Rat Spinal Cord. Primary Antibody: Rabbit Anti-VAChT Antibody at 1:100 for 30min at RT. Secondary Antibody: Anti-Rabbit Poly-HRP-IgG. Ready to use 8 min at RT. Antigen Retrieval: HIER using Citrate Buffer for 20 min. Counterstain: Hematoxylin.