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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- H00006690-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00006690-M01, RRID:AB_489966
- Product name
- SPINK1 monoclonal antibody (M01), clone 4D4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant SPINK1.
- Antigen sequence
DSLGREAKCYNELNGCTKIYDPVCGTDGNTYPNEC
VLCFENRKRQTSILIQKSGPC- Isotype
- IgG
- Antibody clone number
- 4D4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references The construction and proliferative effects of a lentiviral vector capable of stably overexpressing SPINK1 gene in human pancreatic cancer AsPC-1 cell line.
Molecular profiling of ETS gene rearrangements in patients with prostate cancer registered in REDEEM clinical trial.
Molecular profiling of ETS and non-ETS aberrations in prostate cancer patients from northern India.
HOXB13 G84E-related familial prostate cancers: a clinical, histologic, and molecular survey.
Loss of SPINK1 expression is associated with unfavorable outcomes in urothelial carcinoma of the bladder after radical cystectomy.
Novel dual-color immunohistochemical methods for detecting ERG-PTEN and ERG-SPINK1 status in prostate carcinoma.
Characterization of ETS gene aberrations in select histologic variants of prostate carcinoma.
The role of SPINK1 in ETS rearrangement-negative prostate cancers.
Zhang J, Wang D, Hu N, Wang Q, Yu S, Wang J
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 May;37(5):5847-55
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 May;37(5):5847-55
Molecular profiling of ETS gene rearrangements in patients with prostate cancer registered in REDEEM clinical trial.
Palanisamy N, Tsodikov A, Yan W, Suleman K, Rittmaster R, Lucia SM, Chinnaiyan AM, Fleshner N, Kunju LP
Urologic oncology 2015 Mar;33(3):108.e5-13
Urologic oncology 2015 Mar;33(3):108.e5-13
Molecular profiling of ETS and non-ETS aberrations in prostate cancer patients from northern India.
Ateeq B, Kunju LP, Carskadon SL, Pandey SK, Singh G, Pradeep I, Tandon V, Singhai A, Goel A, Amit S, Agarwal A, Dinda AK, Seth A, Tsodikov A, Chinnaiyan AM, Palanisamy N
The Prostate 2015 Jul 1;75(10):1051-62
The Prostate 2015 Jul 1;75(10):1051-62
HOXB13 G84E-related familial prostate cancers: a clinical, histologic, and molecular survey.
Smith SC, Palanisamy N, Zuhlke KA, Johnson AM, Siddiqui J, Chinnaiyan AM, Kunju LP, Cooney KA, Tomlins SA
The American journal of surgical pathology 2014 May;38(5):615-26
The American journal of surgical pathology 2014 May;38(5):615-26
Loss of SPINK1 expression is associated with unfavorable outcomes in urothelial carcinoma of the bladder after radical cystectomy.
Rink M, Park K, Volkmer BG, Xylinas E, Hansen J, Cha EK, Robinson BD, Hautmann R, Küfer R, Engel O, Chun FK, Dahlem R, Rubin MA, Shariat SF, Mosquera JM
Urologic oncology 2013 Nov;31(8):1716-24
Urologic oncology 2013 Nov;31(8):1716-24
Novel dual-color immunohistochemical methods for detecting ERG-PTEN and ERG-SPINK1 status in prostate carcinoma.
Bhalla R, Kunju LP, Tomlins SA, Christopherson K, Cortez C, Carskadon S, Siddiqui J, Park K, Mosquera JM, Pestano GA, Rubin MA, Chinnaiyan AM, Palanisamy N
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2013 Jun;26(6):835-48
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2013 Jun;26(6):835-48
Characterization of ETS gene aberrations in select histologic variants of prostate carcinoma.
Han B, Mehra R, Suleman K, Tomlins SA, Wang L, Singhal N, Linetzky KA, Palanisamy N, Zhou M, Chinnaiyan AM, Shah RB
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2009 Sep;22(9):1176-85
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2009 Sep;22(9):1176-85
The role of SPINK1 in ETS rearrangement-negative prostate cancers.
Tomlins SA, Rhodes DR, Yu J, Varambally S, Mehra R, Perner S, Demichelis F, Helgeson BE, Laxman B, Morris DS, Cao Q, Cao X, Andrén O, Fall K, Johnson L, Wei JT, Shah RB, Al-Ahmadie H, Eastham JA, Eggener SE, Fine SW, Hotakainen K, Stenman UH, Tsodikov A, Gerald WL, Lilja H, Reuter VE, Kantoff PW, Scardino PT, Rubin MA, Bjartell AS, Chinnaiyan AM
Cancer cell 2008 Jun;13(6):519-28
Cancer cell 2008 Jun;13(6):519-28
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SPINK1 monoclonal antibody (M01), clone 4D4. Western Blot analysis of SPINK1 expression in human pancreas.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged SPINK1 is 0.03 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of SPINK1 transfected lysate using anti-SPINK1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with SPINK1 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to SPINK1 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 1 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
