11943-2-AP

antibody from Invitrogen Antibodies

Targeting: PSMA4 HC9, HsT17706

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11943-2-AP

Provider

Invitrogen Antibodies

Product name

PSMA4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MEAIGHAGT CLGILANDGV LLAAERRNIH KLLDEVFFSE KIYKLNEDMA CSVAGITSDA NVLTNELRLI AQRYLLQYQE PIPCEQLVTA LCDIKQAYTQ FGGKRPFGVS LLYIGWDKHY GFQLYQSDPS GNYGGWKATC IGNNSAAAVS MLKQDYKEGE MTLKSALALA IKVLNKTMDV SKLSAEKVEI ATLTRENGKT VIRVLKQKEV EQLIKKHEEE EAKAEREKKE KEQKEKDK (1-237 aa encoded by BC056249)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.33 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11943-2-AP (PSMA4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 11943-2-AP ( PSMA4 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

U-937 cells were subjected to SDS PAGE followed by western blot with 11943-2-AP (PSMA4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11943-2-AP (PSMA4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11943-2-AP (PSMA4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 11943-2-AP (PSMA4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 11943-2-AP ( PSMA4 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 11943-2-AP ( PSMA4 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PSMA4 (IP:11943-2-AP, 4ug; Detection:11943-2-AP 1:500) with BxPC-3 cells lysate 2800ug.