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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00064098-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00064098-M01, RRID:AB_463942
- Product name
- PARVG monoclonal antibody (M01), clone 4E1
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant PARVG.
- Antigen sequence
MEPEFLYDLLQLPKGVEPPAEEELSKGGKKKYLPP
TSRKDPKFEELQKVLMEWINATLLPEHIVVRSLEE
DMFDGLILHHLFQRLAALKLEAEDIALTATSQKHK
LTVVLEAVNRSLQLEEWQAKWSVESIFNKDLLSTL
HLLVALAKRFQPDLSLPTNVQVEVITIESTKSGLK
SEKLVEQLTEYSTDKDEPPKDVFDELFKLAPEKVN
AVKEAIVNFVNQKLDRLGLSVQNLDTQFADGVILL
LLIGQLEGFFLHLKEFYLTPNSPAEMLHNVTLALE
LLKDEGLLSCPVSPEDIVNKDAKSTLRVLYGLFCK
HTQKAHRDRTPHGAPN- Isotype
- IgG
- Antibody clone number
- 4E1
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PARVG monoclonal antibody (M01), clone 4E1 Western Blot analysis of PARVG expression in HL-60 ( Cat # L014V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PARVG expression in transfected 293T cell line by PARVG monoclonal antibody (M01), clone 4E1.Lane 1: PARVG transfected lysate (Predicted MW: 37.5 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PARVG is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of PARVG transfected lysate using anti-PARVG monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PARVG monoclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
