sc-259

antibody from Santa Cruz Biotechnology

Targeting: SOS1 GF1, GINGF, HGF

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-259

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-259, RRID:AB_2270724

Product name

Anti-SOS1

Antibody type

Polyclonal (Antigen purified)

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

References

Submitted references

TGFbeta1 signaling via alphaVbeta6 integrin.

Kracklauer MP, Schmidt C, Sclabas GM
Molecular cancer 2003 Aug 7;2:28

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Ras-guanine nucleotide exchange factor sos2 is dispensable for mouse growth and development.

Esteban LM, Fernández-Medarde A, López E, Yienger K, Guerrero C, Ward JM, Tessarollo L, Santos E
Molecular and cellular biology 2000 Sep;20(17):6410-3

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M2 alpha-1-antitrypsin phenotype and primary liver cancer

P Sizaret, M Clerc, J Estève, R R Frants, J Pillot
British Journal of Cancer 1981 Feb;43(2):226-228

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

No bands detected.

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 128

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows distinct cytoplasmic positivity in neuronal cells.

Validation comment

Staining pattern partly consistent with experimental and/or bioinformatic data.