Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-117671 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SOS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody has been tested in direct-ELISA
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 2.885 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SOS1 using SOS1 Polyclonal Antibody (Product # PA5-117671) at 4.6 µg/mL. Positive WB detected in: Rat brain tissue. Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 153, 37 kDa. Observed band size: 153 kDa
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of SOS1 in HeLa cells using SOS1 Polyclonal Antibody (Product # PA5-117671) diluted at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immnohistochemistry (Paraffin) analysis of SOS1 in paraffin-embedded human colon cancer using SOS1 Polyclonal Antibody (Product # PA5-117671) diluted at 1:400. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immnohistochemistry (Paraffin) analysis of SOS1 in paraffin-embedded human placenta tissue using SOS1 Polyclonal Antibody (Product # PA5-117671) diluted at 1:400. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.