LS-C676253

antibody from LSBio

Targeting: PGLS 6PGL

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C676253

Provider

LSBio

Product name

PGLS / 6PGL Antibody LS-C676253

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating PGLS in U251 whole cell lysate Lane 1: Rabbit monoclonal IgG(1ug)instead of product in U251 whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000) Lane 2: product(4ug)+ U251 whole cell lysate(500ug) Lane 3: U251 whole cell lysate (20ug)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating PGLS in U251 whole cell lysate Lane 1: Rabbit monoclonal IgG(1ug)instead of product in U251 whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000) Lane 2: product(4ug)+ U251 whole cell lysate(500ug) Lane 3: U251 whole cell lysate (20ug)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating PGLS in U251 whole cell lysate Lane 1: Rabbit monoclonal IgG(1ug)instead of product in U251 whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000) Lane 2: product(4ug)+ U251 whole cell lysate(500ug) Lane 3: U251 whole cell lysate (20ug)