Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00025796-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00025796-M01, RRID:AB_2161694
- Product name
- Mouse Monoclonal PGLS Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Reacts with 6-phosphogluconolactonase.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PGLS Antibody (2D9) [H00025796-M01] - Analysis of PGLS expression in transfected 293T cell line by PGLS monoclonal antibody (M01), clone 2D9.Lane 1: PGLS transfected lysate(27.5 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: PGLS Antibody (2D9) [H00025796-M01] - Detection limit for recombinant GST tagged PGLS is 1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: PGLS Antibody (2D9) [H00025796-M01] - Analysis of monoclonal antibody to PGLS on HeLa cell . Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PGLS Antibody (2D9) [H00025796-M01] - Analysis of PGLS transfected lysate using anti-PGLS monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGLS MaxPab rabbit polyclonal antibody.