- PA5-34666 - Invitrogen Antibodies MSN antibody

No submitted references: Submit reference

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Moesin Polyclonal Antibody (Product # PA5-34666). Sample (30 µg of whole cell lysate). Lane A: NT2D1. 7.5% SDS PAGE. Moesin Polyclonal Antibody (Product # PA5-34666) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Moesin Polyclonal Antibody detects MSN protein by Western blot analysis. A. 30 µg GL261 whole cell lysate/extract. B. 30 µg C8D30 whole cell lysate/extract. C. 30 µg NIH-3T3 whole cell lysate/extract. D. 30 µg BCL-1 whole cell lysate/extract. E. 30 µg Raw264.7 whole cell lysate/extract. F. 30 µg C2C12 whole cell lysate/extract.7.5 % SDS-PAGE. Moesin Polyclonal Antibody (Product # PA5-34666) dilution: 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Moesin was achieved by transfecting A-431 cells with Moesin specific siRNAs (Silencer® select Product # s8986, s8984). Western blot analysis (Fig. a) was performed using whole cell extracts from the Moesin knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Moesin Polyclonal Antibody (Product # PA5-34666, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Moesin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), A-431 (Lane 2), Jurkat (Lane 3), COS-7 (Lane 4), C2C12 (Lane 5), NIH/3T3 (Lane 6) and Mouse Spleen (Lane 7). The blot was probed with Anti-Moesin Polyclonal Antibody (Product # PA5-34666, 1:2500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 68kDa band corresponding to Moesin was observed across all cell lines and tissue lysate tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Moesin in methanol-fixed A431 cells using a Moesin polyclonal antibody (Product # PA5-34666) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (red) at a 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Moesin was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Moesin Polyclonal Antibody (Product # PA5-34666) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of Moesin was performed in paraffin-embedded rat spleen tissue using Moesin Polyclonal Antibody (Product # PA5-34666) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of Moesin was performed in A431 whole cell extracts using 5 µg of Moesin Polyclonal Antibody (Product # PA5-34666). Samples were transferred to a membrane and probed with Moesin Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.

PA5-34666