634504

antibody from BioLegend

Targeting: PDCD6IP AIP1, Alix, Hp95

Immunocytochemistry (Data presented on provider website)

Immunoelectron microscopy (Supportive data in Antibodypedia)

Antibody Data

Product number

634504

Provider

BioLegend

Proper citation

BioLegend Cat#634504, RRID:AB_2565615

Product name

Alexa Fluor® 594 anti-Alix

Antibody type

Monoclonal

Antigen

Recombinant full-length human Alix protein

Description

Alix (ALG-2-interacting protein X) is an adaptor protein that was first described for its capacity to bind to the calcium-binding protein ALG-2, the expression of which seemed necessary for cell death. The predicted molecular weight is approximately 96 kD. Alix contains an N-terminal Bro1 domain and a C-terminal proline-rich domain (PRD). The PRD contains multiple polyproline motifs, which are potential docking sites for proteins containing SH3 domains, interact with multiple cellular Alix-binding partners that are involved in apoptotic induction, endosomal sorting, and endocytosis. The Bro1 domain interacts with CHMP4b, which is also involved in endosomal sorting.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

3A9

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunoelectron microscopy

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

NIT/3T3 cells were fixed with -20°C Methanol for ten minutes and then blocked with 5% FBS for 30 minutes. The cells were stained with 5 µg/ml of anti-Alix (Clone 3A9) Alexa Fluor® 594 (red) in blocking buffer overnight at 4°C. Nuclei were counterstained with DAPI and are shown in blue. The image was captured with a 40X objective.

Conjugate

Yellow dye