M01751-1

antibody from Boster Biological Technology

Targeting: PDCD6IP AIP1, Alix, Hp95

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

M01751-1

Provider

Boster Biological Technology

Product name

Anti-ALIX/PDCD6IP Antibody Picoband™(monoclonal, 14D10)

Antibody type

Monoclonal

Description

Mouse IgG monoclonal antibody for ALIX/PDCD6IP detection. Tested with WB, IHC-P, FCM, ICC/IF in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

14D10

Vial size

100μg/vial

Storage

At -20ËšC for one year. After reconstitution, at 4ËšC for one month. It can also be aliquoted and stored frozen at -20ËšC for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Flow cytometry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Flow Cytometry analysis of A431 cells using anti- PDCD6IPantibody (M01751-1).Overlay histogram showing A431 cells stained with M01751-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- PDCD6IP Antibody (M01751-1, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Additional image