12422-1-AP

antibody from Invitrogen Antibodies

Targeting: PDCD6IP AIP1, Alix, Hp95

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12422-1-AP

Provider

Invitrogen Antibodies

Product name

PDCD6IP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: SHRDTIVLL CKPEPELNAA IPSANPAKTM QGSEVVNVLK SLLSNLDEVK KEREGLENDL KSVNFDMTSK FLTALAQDGV INEEALSVTE LDRVYGGLTT KVQESLKKQE GLLKNIQVSH QEFSKMKQSN NEANLREEVL KNLATAYDNF VELVANLKEG TKFYNELTEI LVRFQNKCSD IVFARKTERD ELLKDLQQSI AREPSAPSIP TPAYQSSPAG GHAPTPPTPA PRTMPPTKPQ PPARPPPPVL PANRAPSATA PSPVGAGTAA PAPSQTPGSA PPPQAQGPPY PTYPGYPGYC QMPMPMGYNP YAYGQYNMPY PPVYHQSPGQ APYPGPQQPS YPFPQPPQQS YYPQQ (515-868 aa encoded by BC020066)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.14 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 12422-1-AP ( PDCD6IP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 12422-1-AP (PDCD6IP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat liver tissue were subjected to SDS PAGE followed by western blot with 12422-1-AP (PDCD6IP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 12422-1-AP (PDCD6IP antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using PDCD6IP antibody 12422-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using (Product # 12422-1-AP) (Alix antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PDCD6IP (IP:12422-1-AP, 3ug; Detection:12422-1-AP 1:500) with Jurkat cells lysate 4000ug.