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LearnMAB7776
antibody from Novus Biologicals
Targeting: FBXW7
AGO, CDC4, FBW7, FBX30, FBXW6, FLJ11071, SEL-10, SEL10
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB7776 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal FBXW7/Cdc4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human FBXW7/Cdc4 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Inactivation of EWS reduces PGC-1α protein stability and mitochondrial homeostasis.
Park JH, Kang HJ, Lee YK, Kang H, Kim J, Chung JH, Chang JS, McPherron AC, Lee SB
Proceedings of the National Academy of Sciences of the United States of America 2015 May 12;112(19):6074-9
Proceedings of the National Academy of Sciences of the United States of America 2015 May 12;112(19):6074-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human FBXW7/Cdc4 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FBXW7/Cdc4 Monoclonal Antibody (Catalog # MAB7776) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FBXW7/Cdc4 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
