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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB2133 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2133, RRID:AB_1578818
- Product name
- PIP5K1C polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PIP5K1C.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Type I gamma phosphatidylinositol phosphate kinase targets and regulates focal adhesions.
Recruitment and regulation of phosphatidylinositol phosphate kinase type 1 gamma by the FERM domain of talin.
Type I phosphatidylinositol-4-phosphate 5-kinases. Cloning of the third isoform and deletion/substitution analysis of members of this novel lipid kinase family.
Ling K, Doughman RL, Firestone AJ, Bunce MW, Anderson RA
Nature 2002 Nov 7;420(6911):89-93
Nature 2002 Nov 7;420(6911):89-93
Recruitment and regulation of phosphatidylinositol phosphate kinase type 1 gamma by the FERM domain of talin.
Di Paolo G, Pellegrini L, Letinic K, Cestra G, Zoncu R, Voronov S, Chang S, Guo J, Wenk MR, De Camilli P
Nature 2002 Nov 7;420(6911):85-9
Nature 2002 Nov 7;420(6911):85-9
Type I phosphatidylinositol-4-phosphate 5-kinases. Cloning of the third isoform and deletion/substitution analysis of members of this novel lipid kinase family.
Ishihara H, Shibasaki Y, Kizuki N, Wada T, Yazaki Y, Asano T, Oka Y
The Journal of biological chemistry 1998 Apr 10;273(15):8741-8
The Journal of biological chemistry 1998 Apr 10;273(15):8741-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The PIP5K1C polyclonal antibody (Cat # PAB2133) is used in Western blot to detect PIP5K1C in HeLa cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PIP5K1C (arrow) using PIP5K1C polyclonal antibody (Cat # PAB2133). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIP5K1C gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with PIP5K1C polyclonal antibody (Cat # PAB2133) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
