Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ARG54940 - Provider product page
- Provider
- Arigo
- Product name
- anti-Chk2 antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 111-141 (N-terminus) of Human CHEK2.
- Description
- Purification with Protein A and immunogen peptide.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 6 months. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
No comments: Submit comment
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 30 µg of 1) MCF-7, 2) HT29, 3) DU145, 4) Jurkat, and 5) HeLa cell lysates stained with ARG54940 anti-Chk2 antibody at 1:500 dilution.Data provided by customer.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of K562,NCI-H460 cell line lysates stained with ARG54940 anti-Chk2 antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HepG2 cell stained with ARG54940 anti-Chk2 antibody (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: formalin fixed and paraffin embedded colon carcinoma stained with ARG54940 anti-Chk2 antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: K562 cells stained with ARG54940 anti-Chk2 antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.
