LS-C392881

antibody from LSBio

Targeting: LSM2 C6orf28, G7b, YBL026W

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C392881

Provider

LSBio

Product name

LSM2 / SnRNP Antibody (aa55-89) LS-C392881

Antibody type

Polyclonal

Description

Protein A purified

Reactivity

Human, Mouse

Host

Rabbit

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

All lanes: Anti-LSM2 Antibody (C-Term) at 1:2000 dilution. Lane 1: 293T/17 whole cell lysate. Lane 2: HeLa whole cell lysate. Lane 3: K562 whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 11 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Antibody staining LSM2 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.