MAB8161

antibody from Abnova Corporation

Targeting: GDF15 MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB8161

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB8161, RRID:AB_10676404

Product name

GDF15 monoclonal antibody, clone 23B3.D2.H5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against synthetic peptide of GDF15 .

Isotype

IgG

Antibody clone number

23B3.D2.H5

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Changes in gene expression contribute to cancer prevention by COX inhibitors.

Baek SJ, Eling TE
Progress in lipid research 2006 Jan;45(1):1-16

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H6D polymorphism in macrophage-inhibitory cytokine-1 gene associated with prostate cancer.

Lindmark F, Zheng SL, Wiklund F, Bensen J, Bälter KA, Chang B, Hedelin M, Clark J, Stattin P, Meyers DA, Adami HO, Isaacs W, Grönberg H, Xu J
Journal of the National Cancer Institute 2004 Aug 18;96(16):1248-54

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using GDF15 monoclonal antibody, clone 23B3D2.H5 (Cat # MAB8161).The blot shows detection of recombinant GDF15 protein present in Pichia pastoris whole cell lysates.Lane 1, yeast cell lysate expressing GDF15 H variant with SUMO expression tag at 36 KDa.Lane 2, yeast cell lysate expressing GDF15 D variant with SUMO expression tag at 36 KDa.Lane 3, yeast cell lysate expressing GDF15 H variant.Lane 4, yeast cell lysate expressing GDF15 D variant.Recombinant GDF15 proteins without SUMO correspond to monomer (15 KDa) and dimer (30 KDa) bands as indicated by the arrowheads. All lysates were run under reducing conditions.Primary antibody was used at a 1 : 1,000 dilution in TBS containg 1% BSA and 0.2% Tween, and reacted overnight at 4°C. For detection, a 1 : 40,000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody was used in Blocking Buffer for Fluorescent Western Blotting for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc™ 4000MP Imaging System.