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- References [2]
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- Western blot [1]
- Flow cytometry [1]
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- Product number
- ABIN238331 - Provider product page
- Provider
- antibodies-online
- Proper citation
- Antibodies-Online Cat#ABIN238331, RRID:AB_10954050
- Product name
- anti-Growth Differentiation Factor 15 (GDF15) (N-Term), (AA 30-194) antibody
- Antibody type
- Monoclonal
- Antigen
- genetic immunisation with cDNA encoding aa30-194 (N-term) of MIC-1 proprotein
- Description
- Protein G
- Reactivity
- Human
- Epitope
- N-Term,AA 30-194
- Isotype
- IgG
- Antibody clone number
- ME-6D10
- Vial size
- 100 μg
- Storage
- short term: 2°C - 8°C, long term: -20°C
- Handling
- Avoid repeated freezing and thawing.
Submitted references Role of macrophage inhibitory cytokine-1 in tumorigenesis and diagnosis of cancer.
MIC-1, a novel macrophage inhibitory cytokine, is a divergent member of the TGF-beta superfamily.
Bauskin AR, Brown DA, Kuffner T, Johnen H, Luo XW, Hunter M, Breit SN
Cancer research 2006 May 15;66(10):4983-6
Cancer research 2006 May 15;66(10):4983-6
MIC-1, a novel macrophage inhibitory cytokine, is a divergent member of the TGF-beta superfamily.
Bootcov MR, Bauskin AR, Valenzuela SM, Moore AG, Bansal M, He XY, Zhang HP, Donnellan M, Mahler S, Pryor K, Walsh BJ, Nicholson RC, Fairlie WD, Por SB, Robbins JM, Breit SN
Proceedings of the National Academy of Sciences of the United States of America 1997 Oct 14;94(21):11514-9
Proceedings of the National Academy of Sciences of the United States of America 1997 Oct 14;94(21):11514-9
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Capillary gel electrophoresis of purified ME-1C4 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: purified ME-1C4 antibody. Proteins were separated by CGE, Agilent 2100 Bioanalyzer. Internal control bands (240 kDa / 7 kDa / 4,5 kDa).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- FACS analysis of BOSC23 cells using ME-6D10. BOSC23 cells were transiently transfected with an expression vector encoding either MIC-1 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of ME-6D10 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with MIC-1 transfected cells.
