GTX22787

antibody from GeneTex

Targeting: HSPA1A HSP70-1, HSPA1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Blocking/Neutralizing (Data presented on provider website)

Gel shift (Data presented on provider website)

Antibody Data

Product number

GTX22787

Provider

GeneTex

Proper citation

GeneTex Cat#GTX22787, RRID:AB_384850

Product name

Hsp70 antibody [5A5]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat, Bovine, Chicken/Avian, Drosophila, Rabbit, Yeast

Host

Mouse

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of HSP70 in 50μg of various cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with HSP70 antibody [5A5] at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a proper secondary antibody. Chemiluminescent detection was performed.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of HSP70 in C6 glioma cells. HSP70 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with HSP70 antibody [5A5] at a dilution of 1:100-1:200 over night at 4 °C, washed with PBS and incubated with a proper secondary antibody. Imges were taken at 60X magnification.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation of HSP70 in HeLa cells. Antigen-antibody complexes were formed by incubating 500£gg whole cell lysate with 2£gg of HSP70 monoclonal antibody overnight on a rocking platform at 4¢XC. The immune complexes were captured on 50£gl Protein A/G Agarose , washed extensively, and eluted. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with HSP70 antibody [5A5] at a dilution of 1:1000 overnight rotating at 4¢XC, washed in TBST, and probed with a proper secondary antibody. Chemiluminescent detection was performed.