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LearnNB110-61582
antibody from Novus Biologicals
Targeting: HSPA1A
HSP70-1, HSPA1
Western blot
ELISA Immunocytochemistry Immunohistochemistry Flow cytometry Immunoelectron microscopyAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- NB110-61582 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-61582, RRID:AB_2119704
- Product name
- Mouse Monoclonal HSP70/HSPA1A Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects approx 70kDa. Does not cross-react with HSC70 (HSP73).
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Drosophila, Guinea Pig, Hamster, Porcine, Rabbit, Sheep, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.2 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Proteostasis regulators as potential rescuers of PMM2 activity.
Regular Exercise Participation Contributes to Better Proteostasis, Inflammatory Profile, and Vasoactive Profile in Patients With Hypertension.
Alpha lipoic acid supplementation ameliorates the wrath of simulated tropical heat and humidity stress in male Murrah buffaloes.
The effects of heat stress and plane of nutrition on metabolism in growing pigs.
Benzo[a]pyrene-mediated toxicity in primary pig bladder epithelial cells: a proteomic approach.
Vilas A, Yuste-Checa P, Gallego D, Desviat LR, Ugarte M, Pérez-Cerda C, Gámez A, Pérez B
Biochimica et biophysica acta. Molecular basis of disease 2020 Jul 1;1866(7):165777
Biochimica et biophysica acta. Molecular basis of disease 2020 Jul 1;1866(7):165777
Regular Exercise Participation Contributes to Better Proteostasis, Inflammatory Profile, and Vasoactive Profile in Patients With Hypertension.
Teixeira M, Gouveia M, Duarte A, Ferreira M, Simões MI, Conceição M, Silva G, Magalhães S, Ferreira R, Nunes A, Vieira SI, Ribeiro F
American journal of hypertension 2020 Feb 22;33(2):119-123
American journal of hypertension 2020 Feb 22;33(2):119-123
Alpha lipoic acid supplementation ameliorates the wrath of simulated tropical heat and humidity stress in male Murrah buffaloes.
Samad HA, Konyak YY, Latheef SK, Kumar A, Khan IA, Verma V, Chouhan VS, Verma MR, Maurya VP, Kumar P, Sarkar M, Singh G
International journal of biometeorology 2019 Oct;63(10):1331-1346
International journal of biometeorology 2019 Oct;63(10):1331-1346
The effects of heat stress and plane of nutrition on metabolism in growing pigs.
Pearce SC, Gabler NK, Ross JW, Escobar J, Patience JF, Rhoads RP, Baumgard LH
Journal of animal science 2013 May;91(5):2108-18
Journal of animal science 2013 May;91(5):2108-18
Benzo[a]pyrene-mediated toxicity in primary pig bladder epithelial cells: a proteomic approach.
Verma N, Pink M, Rettenmeier AW, Schmitz-Spanke S
Journal of proteomics 2013 Jun 24;85:53-64
Journal of proteomics 2013 Jun 24;85:53-64
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HSP70/HSPA1A Antibody (C92F3A-5) [NB110-61582] - analysis of Human cell lysates from various cell lines showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: HSP70/HSPA1A Antibody (C92F3A-5) [NB110-61582] - analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 . Tissue: colon carcinoma. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (C92F3A-5) [NB110-61582] - Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (C92F3A-5) [NB110-61582] - Tissue: Heat Shocked Melanoma cells. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 16 hours at RT. Secondary Antibody: Biotin Goat Anti-Mouse. Courtesy of: Dr. Ewa Malusecka, Maria Sklodowska-Curie Memorial Cancer Ceter and Inst. Of Oncology, Poland.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: HSP70/HSPA1A Antibody (C92F3A-5) [NB110-61582] - Fluorescence Activated Cell Sorting analysis using Mouse Anti-Hsp70: FITC Monoclonal Antibody, Clone C92 (SMC-100). Tissue: Heat Shocked CD3+ CD8+ T cells . Species: Mouse. Primary Antibody: Mouse Anti-Hsp70: FITC Monoclonal Antibody (SMC-100) at 1:1000. Courtesy of: Cheryl Cameron, Vaccine and Gene Therapy Instit. Florida.
