200-301-A27

antibody from Rockland Immunochemicals, Inc.

Targeting: HSPA1A HSP70-1, HSPA1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

200-301-A27

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#200-301-A27, RRID:AB_2119386

Product name

Anti-Hsp70 (MOUSE) Monoclonal Antibody - 200-301-A27

Antibody type

Monoclonal

Vial size

200 µl

References

Submitted references

USP5 Is Dispensable for Monoubiquitin Maintenance in Drosophila.

Ristic G, Tsou WL, Guzi E, Kanack AJ, Scaglione KM, Todi SV
The Journal of biological chemistry 2016 Apr 22;291(17):9161-72

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Nrf2 and HSF-1 Pathway Activation via Hydroquinone-Based Proelectrophilic Small Molecules is Regulated by Electrochemical Oxidation Potential.

Satoh T, Stalder R, McKercher SR, Williamson RE, Roth GP, Lipton SA
ASN neuro 2015 Jul-Aug;7(4)

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Dual neuroprotective pathways of a pro-electrophilic compound via HSF-1-activated heat-shock proteins and Nrf2-activated phase 2 antioxidant response enzymes.

Satoh T, Rezaie T, Seki M, Sunico CR, Tabuchi T, Kitagawa T, Yanagitai M, Senzaki M, Kosegawa C, Taira H, McKercher SR, Hoffman JK, Roth GP, Lipton SA
Journal of neurochemistry 2011 Nov;119(3):569-78

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's Protein G purified anti-Hsp70 antibody shows detection of Hsp70 in whole cell lysates from heat shocked HeLa cells. The band marked by the arrowhead corresponds to Hsp70 at an approximate molecular weight of 70 kDa. The primary antibody was used at a 1:1000 dilution.

Validation comment

Western Blot