Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Immunocytochemistry [3]
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Validation data
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- Product number
- ADI-SPA-820-D - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ADI-SPA-820-D, RRID:AB_2039253
- Product name
- HSC70/HSP70 monoclonal antibody (N27F3-4)
- Antibody type
- Monoclonal
- Antigen
- Purified from natural sources
- Description
- Protein G affinity purified.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Guinea Pig, Hamster, Porcine, Rabbit, Sheep, Simian, Xenopus
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- N27F3-4
- Vial size
- 50 μg
- Storage
- -20°C
Submitted references Identification and characterization of a heat-inducible Hsp70 gene from Sorghum bicolor which confers tolerance to thermal stress.
Autophagy supports survival and phototransduction protein levels in rod photoreceptors.
High levels of heat shock protein Hsp72 in cancer cells suppress default senescence pathways.
Co-translational folding of caspase-activated DNase with Hsp70, Hsp40, and inhibitor of caspase-activated DNase.
Heat shock protein 70 moderately enhances peptide binding and transport by the transporter associated with antigen processing.
Heat shock protein 70 is able to prevent heat shock-induced resistance of target cells to CTL.
Hormone and antihormone induce distinct conformational changes which are central to steroid receptor activation.
Binding of heat shock proteins to the avian progesterone receptor.
Autoantibodies to the constitutive 73-kD member of the hsp70 family of heat shock proteins in systemic lupus erythematosus.
Mulaudzi-Masuku T, Mutepe RD, Mukhoro OC, Faro A, Ndimba B
Cell stress & chaperones 2015 Sep;20(5):793-804
Cell stress & chaperones 2015 Sep;20(5):793-804
Autophagy supports survival and phototransduction protein levels in rod photoreceptors.
Zhou Z, Doggett TA, Sene A, Apte RS, Ferguson TA
Cell death and differentiation 2015 Mar;22(3):488-98
Cell death and differentiation 2015 Mar;22(3):488-98
High levels of heat shock protein Hsp72 in cancer cells suppress default senescence pathways.
Yaglom JA, Gabai VL, Sherman MY
Cancer research 2007 Mar 1;67(5):2373-81
Cancer research 2007 Mar 1;67(5):2373-81
Co-translational folding of caspase-activated DNase with Hsp70, Hsp40, and inhibitor of caspase-activated DNase.
Sakahira H, Nagata S
The Journal of biological chemistry 2002 Feb 1;277(5):3364-70
The Journal of biological chemistry 2002 Feb 1;277(5):3364-70
Heat shock protein 70 moderately enhances peptide binding and transport by the transporter associated with antigen processing.
Chen D, Androlewicz MJ
Immunology letters 2001 Jan 1;75(2):143-8
Immunology letters 2001 Jan 1;75(2):143-8
Heat shock protein 70 is able to prevent heat shock-induced resistance of target cells to CTL.
Dressel R, Elsner L, Quentin T, Walter L, Günther E
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 1;164(5):2362-71
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 1;164(5):2362-71
Hormone and antihormone induce distinct conformational changes which are central to steroid receptor activation.
Allan GF, Leng X, Tsai SY, Weigel NL, Edwards DP, Tsai MJ, O'Malley BW
The Journal of biological chemistry 1992 Sep 25;267(27):19513-20
The Journal of biological chemistry 1992 Sep 25;267(27):19513-20
Binding of heat shock proteins to the avian progesterone receptor.
Kost SL, Smith DF, Sullivan WP, Welch WJ, Toft DO
Molecular and cellular biology 1989 Sep;9(9):3829-38
Molecular and cellular biology 1989 Sep;9(9):3829-38
Autoantibodies to the constitutive 73-kD member of the hsp70 family of heat shock proteins in systemic lupus erythematosus.
Minota S, Cameron B, Welch WJ, Winfield JB
The Journal of experimental medicine 1988 Oct 1;168(4):1475-80
The Journal of experimental medicine 1988 Oct 1;168(4):1475-80
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis of HSC70/HSP70, mAb (N27F3-4) (Prod. No. ADI-SPA-820): Lane 1: MW marker; Lane 2: HSC70/HSP73 (bovine), (recombinant) (Prod. No. ADI-SPP-751); Lane 3: HSP70/HSP72 (human), (recombinant) (Prod. No. ADI-NSP-555); Lane 4: 3T3; Lane 5: 3T3 (heat shocked); Lane 6: HeLa; Lane 7: HeLa (heat shocked); Lane 8: PC-12; Lane 9: PC-12 (heat shocked).
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of human spleen tissue stained with HSC70/HSP70, mAb (N27F3-4) at 10µg/ml.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Flow cytometry analysis of human colon cancer Caco2 cells analyzed by flow cytometry using isotype control.