PA5-36790

antibody from Invitrogen Antibodies

Targeting: CSNK1A1 CK1, CK1a, CK1alpha, CKIa, CKIalpha

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-36790

Provider

Invitrogen Antibodies

Product name

Phospho-CK1 alpha (Thr321) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects endogenous protein at a molecular weight of 42 kDa.

Concentration

1 mg/mL

References

Submitted references

Methazolamide Attenuates the Development of Diabetic Cardiomyopathy by Promoting β-Catenin Degradation in Type 1 Diabetic Mice.

Chen X, Li Y, Yuan X, Yuan W, Li C, Zeng Y, Lian Y, Qiu X, Qin Y, Zhang G, Liu X, Luo C, Luo JD, Hou N
Diabetes 2022 Apr 1;71(4):795-811

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A Brain Signaling Framework for Stress-Induced Depression and Ketamine Treatment Elucidated by Phosphoproteomics.

Xiao Y, Luo H, Yang WZ, Zeng Y, Shen Y, Ni X, Shi Z, Zhong J, Liang Z, Fu X, Tu H, Sun W, Shen WL, Hu J, Yang J
Frontiers in cellular neuroscience 2020;14:48

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-CK1 alpha (Thr321) in Lane 1: HEK293T cell lysate treated with UV (4h), Lane 2: mouse liver tissue lysate, Lane 3: H9C2 cell lysate treated with UV (4h). Samples were incubated with Phospho-CK1 alpha (Thr321) polyclonal antibody (Product # PA5-36790) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-Casein Kinase I alpha pThr321 using Phospho-Casein Kinase I alpha pThr321 polyclonal antibody (Product # PA5-36790) at a dilution of 1:500. Lane 1: HEK293T cell lysate treated with UV (4h), Lane 2: Mouse liver tissue lysate, Lane 3: H9C2 cell lysate treated with UV (4h).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 Candidate verification by RNA in situ or western blot. (A-E) RNAscope in situ hybridization was performed on coronal sections of wild-type mouse brains in the mPFC and NAc with a Prkcg probe. Prkcg mRNA was expressed in both the mPFC and the NAc. Scale bars are 200 mum. B, bregma; cg1, cingulate cortex, area 1; PrL, prelimbic cortex; IL, infralimbic cortex; aca, anterior commissure, anterior part; AcbC, accumbens nucleus, core; LAcbSh, lateral accumbens shell; AcbSh, accumbens nucleus, shell; DEn, dorsal endopiriform claustrum; IEn, intermediate endopiriform claustrum; VP, ventral pallidum. (F-G) Western blot analysis for the phosphorylation of Csnk1a1 at T321. All data are shown as mean +-SEM. * P < 0.05, one-way ANOVA tests for CUMS + saline compared with Control + saline; ** P < 0.01, one-way ANOVA tests for CUMS + saline compared with CUMS + ketamine.