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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- ELISA [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- H00064399-M01 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HHIP Monoclonal Antibody (5D11)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: GDAKFGERNE GSGARRRRCL NGNPPKRLKR RDRRMMSQLE LLSGGEMLCG GFYPRLSCCL RSDSPGLGRL ENKIFSVTNN TECGKLLEEI KCALCSPHSQ
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5D11
- Vial size
- 100 µg
- Concentration
- See Label
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HHIP expression in transfected 293T cell line by HHIP monoclonal antibody (M01), clone 5D11.Lane 1: HHIP transfected lysate(78.9 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HHIP monoclonal antibody (M01), clone 5D11. Western Blot analysis of HHIP expression in MCF-7 (Cat # L046V1).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HHIP expression in transfected 293T cell line by HHIP monoclonal antibody (M01), clone 5D11.Lane 1: HHIP transfected lysate(78.9 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HHIP monoclonal antibody (M01), clone 5D11 Western Blot analysis of HHIP expression in HeLa S3 NE (Cat # L013V3).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged HHIP is approximately 1 ng/mL as a capture antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to HHIP on formalin-fixed paraffin-embedded human heart. Antibody concentration is 3 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of HHIP transfected lysate using anti-HHIP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HHIP MaxPab rabbit polyclonal antibody.
