- PA5-21705 - Invitrogen Antibodies SUOX antibody

Submitted references Increased hippocampal excitability in miR-324-null mice.

Hayman DJ, Modebadze T, Charlton S, Cheung K, Soul J, Lin H, Hao Y, Miles CG, Tsompani D, Jackson RM, Briggs MD, Piróg KA, Clark IM, Barter MJ, Clowry GJ, LeBeau FEN, Young DA
Scientific reports 2021 May 17;11(1):10452

Diabetic nephropathy associates with deregulation of enzymes involved in kidney sulphur metabolism.

Uyy E, Suica VI, Boteanu RM, Safciuc F, Cerveanu-Hogas A, Ivan L, Stavaru C, Simionescu M, Antohe F
Journal of cellular and molecular medicine 2020 Oct;24(20):12131-12140

Extramitochondrial cardiolipin suggests a novel function of mitochondria in spermatogenesis.

Ren M, Xu Y, Erdjument-Bromage H, Donelian A, Phoon CKL, Terada N, Strathdee D, Neubert TA, Schlame M
The Journal of cell biology 2019 May 6;218(5):1491-1502

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western Blot using SUOX Polyclonal Antibody (Product # PA5-21705). Sample (30 µg whole cell lysate). A: Hep G2. 7.5% SDS PAGE. SUOX Polyclonal Antibody (Product # PA5-21705) diluted at 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using SUOX Polyclonal Antibody (Product # PA5-21705). Sample (50 µg of whole cell lysate). Lane A: mouse liver . 7.5% SDS PAGE. SUOX Polyclonal Antibody (Product # PA5-21705) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescent analysis of SUOX in methanol-fixed HepG2 cells using a SUOX polyclonal antibody (Product # PA5-21705) at a 1:200 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of methanol-fixed HepG2, using SUOX antibody (Product # PA5-21705) at 1:200 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using SUOX (Product # PA5-21705) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: FIGURE 4 Changes in protein abundance induced by diabetes implicated in KEGG sulphur metabolism pathway and H 2 S production. (A) The proteins involved in sulphate activation and degradation, in clearance and H 2 S endogenous production are separately represented. Note that the following enzymes are up-regulated in diabetic kidney of double transgenic ( dTg) samples vs wild-type (WT) renal homogenates: Papss2 (by ~1.7-fold, P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 7 Identification of novel miR-324-5p targets using 3'UTR luciferase assays. ( a ) C3H10T1/2 murine cells were transfected with 3'UTR-pmiRGLO constructs and subsequently also with either a negative control miRNA mimic (miCon) or a mimic of miR-324-5p or miR-324-3p. Luciferase activity was measured after cells were incubated at 37 degC for 24 h. Relative luciferase activity was calculated as a ratio of renilla activity, an internal transfection control of pmiRGLO, and values were plotted as a percentage of mean miCon luciferase activity for each construct. Suox and Cd300lf , but not Sp6 , were identified as direct miR-324-5p targets in vitro. ( b ) When 2nts of the miR-324 binding sites of the Cd300lf and Suox 3'UTRs were mutated, shown as Cd300lf(mut) and Suox(mut) , the miR-324-5p-mediated repression was inhibited in both cases. The predicted miR-324-5p binding sites of each 3'UTR are shown above the panel corresponding to that 3'UTR, with mutations highlighted in black. For panels a and b , the means of at least 3 independent experiments were used to test statistical significance in two-tailed Student's t -tests. A negative control was included for each independent experiment (pmiRGLO) in addition to miCon and, after miR-324 targets were identified, one of these was used as a positive control for each independent experiment. ( c ) Western Blotting was used to confirm that the changes to the quantity of target gene mRNAs affected levels of the corresponding proteins in

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 4. The acrosome contains mitochondrial proteins. (a) Orthodox and condensed mitochondria were separated by density gradient and affinity purified. The organelles were analyzed by TMT proteomics technology. Logarithmic MS1 signal intensities were plotted against the logarithmic ratio of the MS2-based intensities of the condensed tag over the orthodox tag. Only mitochondrial proteins are shown. The data were skewed toward negative log2 ratios because mitochondrial proteins were relatively underrepresented in condensed mitochondria. Proteins with a z-score less than -1 (specific for orthodox mitochondria) and proteins with a z-score greater than +1 (specific for condensed mitochondria) were analyzed with regard to their submitochondrial localization. OM, outer membrane; IMS, intermembrane space. (b) Sperm were analyzed by immunofluorescence by using commercial antibodies to Izumo1 (acrosome marker), Ant4, and the SdhA subunit of respiratory complex II. Hoechst dye was used to stain the nucleus. (c) Sperm heads were analyzed by immunofluorescence by using antibodies to Izumo1, Ant4, Spata18, and Suox.

PA5-21705

Antibody data