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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 67967-1-IG150UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HADHB Monoclonal Antibody (1D12F4)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1D12F4
- Vial size
- 150 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates (pig heart, Rabbit heart s, Rat heart s, Mouse heart s) were subjected to SDS PAGE followed by western blot with (Product # 67967-1-IG150UL) (HADHB antibody) at dilution of 1:20,000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HADHB antibody ((Product # 67967-1-IG150UL), Clone: 1D12F4 ) at dilution of 1:400 and CoraLite 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using (Product # 67967-1-IG150UL) (HADHB antibody) at dilution of 1:2,000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using (Product # 67967-1-IG150UL) (HADHB antibody) at dilution of 1:2,000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
