66238-1-IG

antibody from Invitrogen Antibodies

Targeting: STXBP2 Hunc18b, UNC18B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

66238-1-IG

Provider

Invitrogen Antibodies

Product name

STXBP2 Monoclonal Antibody (3G3A12)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: LHELTFQAM AYDLLDIEQD TYRYETTGLS EAREKAVLLD EDDDLWVELR HMHIADVSKK VTELLRTFCE SKRLTTDKAN IKDLSQILKK MPQYQKELNK YSTHLHLADD CMKHFKGSVE KLCSVEQDLA MGSDAEGEKI KDSMKLIVPV LLDAAVPAYD KIRVLLLYIL LRNGVSEENL AKLIQHANVQ AHSSLIRNLE QLGGTVTNPG GSGTSSRLEP RERMEPTYQL SRWTPVIKDV MEDAVEDRLD RNLWPFVSDP APTASSQAAV SARFGHWHKN KAGIEARAGP RLIVYVMGGV AMSEMRAAYE VTRATEGKWE VLIGSSHILT PTRFLDDLKA LDKKLEDIAL P (241-590 aa encoded by BC002869)

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

3G3A12

Vial size

150 µL

Concentration

1.5 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66238-1-Ig (STXBP2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 66238-1-IG ( STXBP2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 66238-1-IG (STXBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 66238-1-IG (STXBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 66238-1-Ig (STXBP2 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 66238-1-Ig (STXBP2 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 66238-1-IG ( STXBP2 Antibody) at dilution of 1:400 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 66238-1-IG ( STXBP2 Antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-STXBP2 (IP:66238-1-IG, 5ug; Detection:66238-1-IG 1:2000) with K-562 cells lysate 3200ug.