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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00005360-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005360-M01, RRID:AB_425599
- Product name
- PLTP monoclonal antibody (M01), clone 2F3-G4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant PLTP.
- Antigen sequence
FPGCKIRVTSKALELVKQEGLRFLEQELETITIPD
LRGKEGHFYYNISEVKVTELQLTSSELDFQPQQEL
MLQITNASLGLRFRRQLLYWFLKVYDFLSTFITSG
MRFLLNQQICPVLYHAGTVLLNSLLDTVPVRSSVD
ELVGIDYSLMKDPVASTSNLDMDFRGAFFPLTERN
WSLPNRAVEPQLQEEERMVYVAFSEFFFDSAMESY
FRAGALQLLLVGDKVPHDLDMLLRATYFGSIVLLS
PAVIDSPLKLELRVLAPPRCTIKPSGTTISVTASV
TIALVPPDQPEVQLSSMTMDARLSAKMALRGKALR
TQLDLRRFRIYSNHSALESLALIPLQAPLKTMLQI
GVMPMLNERTWRGVQIPLPEGINFVHEVVTNHAGF
LTIGADLHFAKGLREVIEKNRPADVRASTAPTPST
AAV- Isotype
- IgG
- Antibody clone number
- 2F3-G4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Increased biglycan in aortic valve stenosis leads to the overexpression of phospholipid transfer protein via Toll-like receptor 2.
Derbali H, Bossé Y, Côté N, Pibarot P, Audet A, Pépin A, Arsenault B, Couture C, Després JP, Mathieu P
The American journal of pathology 2010 Jun;176(6):2638-45
The American journal of pathology 2010 Jun;176(6):2638-45
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PLTP monoclonal antibody (M01), clone 2F3-G4 Western Blot analysis of PLTP expression in Hela S3 NE ( Cat # L013V3 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PLTP expression in transfected 293T cell line by PLTP monoclonal antibody (M01), clone 2F3-G4.Lane 1: PLTP transfected lysate(54.7 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PLTP is 0.3 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to PLTP on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue. [antibody concentration 5 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
