- NB400-106 - Novus Biologicals PLTP antibody

Submitted references PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity.

Audo R, Deckert V, Daien CI, Che H, Elhmioui J, Lemaire S, Pais de Barros JP, Desrumaux C, Combe B, Hahne M, Lagrost L, Morel J
PloS one 2018;13(3):e0193815

Attenuation of atherogenic apo B-48-dependent hyperlipidemia and high density lipoprotein remodeling induced by vitamin C and E combination and their beneficial effect on lethal ischemic heart disease in mice.

Contreras-Duarte S, Chen P, Andía M, Uribe S, Irarrázaval P, Kopp S, Kern S, Marsche G, Busso D, Wadsack C, Rigotti A
Biological research 2018 Sep 15;51(1):34

Phospholipid transfer protein and alpha-1 antitrypsin regulate Hck kinase activity during neutrophil degranulation.

Ochieng P, Nath S, Macarulay R, Eden E, Dabo A, Campos M, Jiang XC, Foronjy RF, Geraghty P
Scientific reports 2018 Oct 18;8(1):15394

Inactivation of Tm6sf2, a Gene Defective in Fatty Liver Disease, Impairs Lipidation but Not Secretion of Very Low Density Lipoproteins.

Smagris E, Gilyard S, BasuRay S, Cohen JC, Hobbs HH
The Journal of biological chemistry 2016 May 13;291(20):10659-76

Plasma phospholipid transfer protein, cholesteryl ester transfer protein and lecithin:cholesterol acyltransferase in end-stage renal disease (ESRD).

Pahl MV, Ni Z, Sepassi L, Moradi H, Vaziri ND
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2009 Aug;24(8):2541-6

Control of cholesteryl ester transfer protein activity by sequestration of lipid transfer inhibitor protein in an inactive complex.

He Y, Greene DJ, Kinter M, Morton RE
Journal of lipid research 2008 Jul;49(7):1529-37

Atheroprotective potential of macrophage-derived phospholipid transfer protein in low-density lipoprotein receptor-deficient mice is overcome by apolipoprotein AI overexpression.

Valenta DT, Ogier N, Bradshaw G, Black AS, Bonnet DJ, Lagrost L, Curtiss LK, Desrumaux CM
Arteriosclerosis, thrombosis, and vascular biology 2006 Jul;26(7):1572-8

A geriatric survey in general practice.

Hay EH
The Practitioner 1976 Apr;216(1294):443-7

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: PLTP Antibody [NB400-106] - WB analysis of PLTP in human brain lysate.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: PLTP Antibody [NB400-106] - Activation of PLTP in STAT3 pathway. RA-FLS were stimulated for 24 hours with rhPLTP at the indicated concentrations. Western blot analyses analyzed cell lysates for phosphorylation of STAT3 (Tyr705). Band intensities were normalized to corresponding STAT3 band intensities. Representative Western blots are shown (n = 3). Citation: Audo R, Deckert V, Daien CI, Che H, Elhmioui J, Lemaire S, et al. (2018) PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity. PLoS ONE 13(3): e0193815.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: PLTP Antibody [NB400-106] - PLTP antibody expression in RA synovial tissues. (A) Immuno-histological analyses used RA patients synovial tissue sections (n=5). They were stained for PLTP or macrophages (CD68+ cells). Blue arrows indicate macrophages (non-exhaustive), determined as CD68+ cells. FLS are determined with CD68+ staining (black arrow; non-exhaustive) and morphological features. (B, C) Double staining displays localization (n = 3) of PLTP with macrophages (CD68+) in B or RA-FLS (alpha-SMA+) in C. Overlay is shown to visualize co-localization of PLTP. All magnification and fluorescence analysis at 20x. Citation: Audo R, Deckert V, Daien CI, Che H, Elhmioui J, Lemaire S, et al. (2018) PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity. PLoS ONE 13(3): e0193815. https://doi.org/10.1371/journal.pone.0193815

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: PLTP Antibody [NB400-106] - PLTP expression and activity in OA and RA joints. (A) Western blot analysis quantified PLTP protein level in FLS and was normalized using beta-actin. Expressed as a ratio with mean expression level in all FLS tested. Representative images obtained from one gel are shown. All data shown as mean +/- SD. Mann-Whitney test used for statistical analyses. (B) Immunohistological analysis of PLTP expression in synovial tissue from 5 RA and 6 OA patients. Tissues were stained for PLTP and macrophages (CD68+ cells); Positive staining in all RA synovial tissue and only 2 out 6 OA tissues. Representative stainings are shown. Citation: Audo R, Deckert V, Daien CI, Che H, Elhmioui J, Lemaire S, et al. (2018) PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity. PLoS ONE 13(3): e0193815.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: PLTP Antibody [NB400-106] - Recombinant PLTP antibody induced cytokine production and FLS proliferation, independent of its lipid transfer ability. (A) RA-FLS were stimulated for 48 hours with PLTP or heat inactivated-PLTP and proliferation was evaluated during the final day of stimulation using thymidine incorporation. (B) Blockade of PLTP decreased the effect of rhPLTP on FLS proliferation. (C) PLTP impact on RA-FLS cytokine production. (D) OA-FLS were treated with either native PLTP or heated-PLTP and analyzed for cytokine production and proliferation. Citation: Audo R, Deckert V, Daien CI, Che H, Elhmioui J, Lemaire S, et al. (2018) PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity. PLoS ONE 13(3): e0193815.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: PLTP Antibody [NB400-106] - PLTP was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human PLTP polyclonal Antibody (Catalog # NB400-106) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in decidual cells.

NB400-106

Antibody data