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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- H00054821-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00054821-M01, RRID:AB_425995
- Product name
- ERCC6L monoclonal antibody (M01), clone 3F12-2B10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant ERCC6L.
- Antigen sequence
MEKSFATKNEAVQKETLQEGPKQEALQEDPLESFN
YVLSKSTKADIGPNLDQLKDDEILRHCNPWPIISI
TNESQNAESNVSIIEIADDLSASHSALQDAQASEA
KLEEEPSASSPQYACDFNLFLEDSADNRQNFSSQS
LEHVEKENSLCGSAPNSRAGFVHSKTCLSWEFSEK
DDEPEEVVVKAKIRSKARRIVSDGEDEDDSFKDTS
SINPFNTSLFQFSSVKQFDASTPKNDISPPGRFFS
SQIPSSVNKSMNSRRSLASRRSLINMVLDHVEDME
ERLDDSSEAKGPEDYPEEGVEESSGEASKYTEEDP
SGETLSSENKSSWLMTSKPSALAQETSLGAPEPLS
GEQLVGSPQDKAAEATNDYETLVKRGKELKECGKI
QEALNCLVKALDIKSADPEVMLLTLSLYKQLNNN- Isotype
- IgG
- Antibody clone number
- 3F12-2B10
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references PICH and cotargeted Plk1 coordinately maintain prometaphase chromosome arm architecture.
Targeting Plk1 to chromosome arms and regulating chromosome compaction by the PICH ATPase.
Kurasawa Y, Yu-Lee LY
Molecular biology of the cell 2010 Apr 1;21(7):1188-99
Molecular biology of the cell 2010 Apr 1;21(7):1188-99
Targeting Plk1 to chromosome arms and regulating chromosome compaction by the PICH ATPase.
Leng M, Bessuso D, Jung SY, Wang Y, Qin J
Cell cycle (Georgetown, Tex.) 2008 May 15;7(10):1480-9
Cell cycle (Georgetown, Tex.) 2008 May 15;7(10):1480-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of ERCC6L expression in transfected 293T cell line by ERCC6L monoclonal antibody (M01), clone 3F12-2B10.Lane 1: ERCC6L transfected lysate(46.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ERCC6L is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
