PA5-78347

antibody from Invitrogen Antibodies

Targeting: EHMT2 BAT8, C6orf30, Em:AF134726.3, G9A, KMT1C, NG36/G9a

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-78347

Provider

Invitrogen Antibodies

Product name

EHMT2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: 293T, A431, HeLa, HepG2, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12

Concentration

1.34 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of G9a in non-transfected (-) and transfected (+) 293T cells using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with G9a polyclonal antibody (Product # PA5-78347) using a dilution of 1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of G9a in A) 293T whole cell lysate, B) A431 whole cell lysate, C) HeLa whole cell lysate, D) HepG2 whole cell lysate using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with G9a polyclonal antibody (Product # PA5-78347) using a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using EHMT2 Polyclonal Antibody (Product # PA5-78347). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with EHMT2 Polyclonal Antibody (Product # PA5-78347) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

EHMT2 Polyclonal Antibody detects G9a protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with EHMT2 Polyclonal Antibody (Product # PA5-78347) diluted at 1:3,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

EHMT2 Polyclonal Antibody detects G9a protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: G9a protein stained by EHMT2 Polyclonal Antibody (Product # PA5-78347) diluted at 1:1,000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

EHMT2 Polyclonal Antibody detects G9a protein at nucleus on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. EHMT2 Polyclonal Antibody (Product # PA5-78347) diluted at 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ChIP was performed with HeLa chromatin extract and 5 µg of either normal rabbit IgG or EHMT2 Polyclonal Antibody (Product # PA5-78347). The precipitated DNA was detected by PCR with primer set targeting to FXN.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

EHMT2 Polyclonal Antibody immunoprecipitates G9a protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : Control with 3 µg of pre-immune rabbit IgG B : Immunoprecipitation of G9a by 3 µg of EHMT2 Polyclonal Antibody (Product # PA5-78347) 5% SDS-PAGE The immunoprecipitated G9a protein was detected by EHMT2 Polyclonal Antibody (Product # PA5-78347) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.