NB100-40825
antibody from Novus Biologicals
Targeting: EHMT2
BAT8, C6orf30, Em:AF134726.3, G9A, KMT1C, NG36/G9a
Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
Reference
Comment
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- Product number
- NB100-40825 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-40825, RRID:AB_2262142
- Product name
- Rabbit Polyclonal G9a/EHMT2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Chemoproteomics profiling of HDAC inhibitors reveals selective targeting of HDAC complexes.
Enhanced expression of EHMT2 is involved in the proliferation of cancer cells through negative regulation of SIAH1.
Enhanced expression of EHMT2 is involved in the proliferation of cancer cells through negative regulation of SIAH1.
Bantscheff M, Hopf C, Savitski MM, Dittmann A, Grandi P, Michon AM, Schlegl J, Abraham Y, Becher I, Bergamini G, Boesche M, Delling M, Dümpelfeld B, Eberhard D, Huthmacher C, Mathieson T, Poeckel D, Reader V, Strunk K, Sweetman G, Kruse U, Neubauer G, Ramsden NG, Drewes G
Nature biotechnology 2011 Mar;29(3):255-65
Nature biotechnology 2011 Mar;29(3):255-65
Enhanced expression of EHMT2 is involved in the proliferation of cancer cells through negative regulation of SIAH1.
Cho HS, Kelly JD, Hayami S, Toyokawa G, Takawa M, Yoshimatsu M, Tsunoda T, Field HI, Neal DE, Ponder BA, Nakamura Y, Hamamoto R
Neoplasia (New York, N.Y.) 2011 Aug;13(8):676-84
Neoplasia (New York, N.Y.) 2011 Aug;13(8):676-84
Enhanced expression of EHMT2 is involved in the proliferation of cancer cells through negative regulation of SIAH1.
Cho HS, Kelly JD, Hayami S, Toyokawa G, Takawa M, Yoshimatsu M, Tsunoda T, Field HI, Neal DE, Ponder BA, Nakamura Y, Hamamoto R
Neoplasia (New York, N.Y.) 2011 Aug;13(8):676-84
Neoplasia (New York, N.Y.) 2011 Aug;13(8):676-84
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: G9a/EHMT2 Antibody [NB100-40825] - Detection of Human G9A/EHMT2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G9A/EHMT2 antibody NB100-40825 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: G9a/EHMT2 Antibody [NB100-40825] - Whole cell lysate (15 and 50 ug) from HeLa cells. Antibody used for WB at 1 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: G9a/EHMT2 Antibody [NB100-40825] - Detection of human G9A/EHMT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G9A/EHMT2 antibody NB100-40825 (lot NB100-40825-2) used for IP at 6 ug per reaction. G9A/EHMT2 was also immunoprecipitated by a previous lot of this antibody (NB100-40825-1) and another rabbit anti-G9A/EHMT2 antibody. For blotting immunoprecipitated G9A/EHMT2, NB100-40825 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: G9a/EHMT2 Antibody [NB100-40825] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-G9A/EHMT2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: G9a/EHMT2 Antibody [NB100-40825] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- G9A/EHMT2 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB