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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
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Validation data
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- Product number
- 20814-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SHD Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAKWLRDYL SFGGRRPPPQ PPTPDYTESD ILRAYRAQKN LDFEDPYEDA ESRLEPDPAG PGDSKNPGDA KYGSPKHRLI KVEAADMARA KALLGGPGEE LEADTEYLDP FDAQPHPAPP DDGYMEPYDA QWVMSELPGR GVQLYDTPYE EQDPETADGP PSGQKPRQSR MPQEDERPAD EYDQPWEWKK DHISRAFAVQ FDSPEWERTP GSAKELRRPP PRSPQPAERV DPALPLEKQP WFHGPLNRAD AESLLSLCKE GSYLVRLSET NPQDCSLSLR SSQGFLHLKF ARTRENQVVL GQHSGPFPSV PELVLHYSSR PLPVQGAEHL ALLYPVVTQT P (1-340 aa encoded by BC007206)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 20814-1-AP (SHD Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 20814-1-AP (SHD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 20814-1-AP ( SHD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neuro-2a cells were subjected to SDS PAGE followed by western blot with 20814-1-AP (SHD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed Neuro-2a cells using 20814-1-AP (SHD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
