21129-1-AP

antibody from Invitrogen Antibodies

Targeting: CEMIP HYBID, IR2155535, KIAA1199, TMEM2L

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

21129-1-AP

Provider

Invitrogen Antibodies

Product name

KIAA1199 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: TVAAGCPDQ SPELQPWNPG HDQDHHVHIG QGKTLLLTSS ATVYSIHISE GGKLVIKDHD EPIVLRTRHI LIDNGGELHA GSALCPFQGN FTIILYGRAD EGIQPDPYYG LKYIGVGKGG ALELHGQKKL SWTFLNKTLH PGGMAEGGYF FERSWGHRGV IVHVIDPKSG TVIHSDRFDT YRSKKESERL VQYLNAVPDG RILSVAVNDE GSRNLDDMAR KAMTKLGSKH FLHLGFRHPW SFLTVKGNPS SSVEDHIEYH GHRGSAAARV FKLFQTEHGE YFNVSLSSEW VQDVEWTEWF DHDKVSQTKG GEKISDLWKA HPGKICNRPI DIQATTMDGV NLSTEVVYKK GQDYRFA (31-386 aa encoded by BC020256)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.3 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

COLO 320 cells were subjected to SDS PAGE followed by western blot with 21129-1-AP (KIAA1199 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SGC-7901 cells were subjected to SDS PAGE followed by western blot with 21129-1-AP (KIAA1199 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using CEMIP antibody 21129-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using KIAA1199 antibody 21129-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate hyperplasia using 21129-1-AP (KIAA1199 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate hyperplasia using 21129-1-AP (KIAA1199 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 21129-1-AP (KIAA1199 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 21129-1-AP (KIAA1199 Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human stomach cancer using 21129-1-AP (CEMIP antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human stomach cancer using 21129-1-AP (CEMIP antibody) at dilution of 1:50 (under 40x lens).